IVF-ET技术发展迅速，但妊娠率远未达到人们期望。生殖免疫学认为移植失败与机体免疫异常有关。抗核抗体（ANA）作为女性人群中最常见的自身抗体，可能与细胞核成分相结合影响细胞分裂，导致不孕或流产。我们既往研究提示，血清、卵泡液中ANA表达影响IVF-ET的结局，阳性组妊娠率降低，流产率增高；另外在子宫内膜分泌物中检出ANA；我们认为血清、卵泡液、子宫内膜中的ANA可能影响成功妊娠。为验证假设，我们对IVF-ET患者的血清、卵泡液、子宫内膜分泌物进行筛查，液态微球芯片法对ANA分型检测，探讨不同表达部位及不同亚型的ANA对IVF-ET的影响；将自身阳性血清、单/多克隆ANA与废弃胚胎及颗粒细胞共培养，液态微球芯片技术检测培养上清液中凋亡因子的表达，免疫荧光法检测废胚及颗粒细胞中ANA结合位点；研究ANA与胚胎靶抗原结合、对胚胎细胞凋亡的影响，以探讨其影响胚胎发育的可能机制。

Although in vitro fertilization and embryo transfer techniques have been rapidly developed in recent years, a portion of infertile patients consistently fail to conceive despite recurrent transfers of high-quality embryos. One of the reason may be the break of the homeostasis in immune system. As a commonly seen autoantibody in women, anti-nuclear antibody targeted the important components in cell nuclear. This effect may impact the zygote division and leads to the failure of pregnant.In our previous study, we found that the presence of ANA in serum predicted an adverse IVF/ICSI outcome, primarily reflecting the reduced rates of implantation and clinical pregnancy, as well as the increased rates of early miscarriage. We hypothesize that ANA in the serum, the follicular fluid and the endometrial secretion may impact IVF-ET treatment by it adverse effect on embryo development and promote effect on basal layer cell and embryo cell damage. To verify our hypothesize, we plan to test and type ANA in serum, follicular fluid and endometrial secretion by indirect immunofluorescence assay and the liquid bead microarray to identify the impact effect of ANA by subtypes in the IVF-ET treatment. Mechanism research will be performed by culture embryos or granular cells with ANA positive serum and commercial ANA. Followed with intracellular apoptosis factors from embryos or granular cells will be tested by liquid bead microarray; immunofluorescence assay will be performed to identify the existence of ANA in the cell. Research data and clinic data will be summarized to study the impact of ANA in embryo development after IVF-ET treatment.