哺乳动物生精上皮周期性变化与血睾屏障功能的维持和精子发生过程密切相关。申请者前期研究发现miR-9/211/142-3p在大鼠睾丸中的表达具有生精周期依赖性，通过生物信息学软件预测其靶基因为Scrib极性蛋白复合体。基于Scrib复合体蛋白在调节上皮细胞功能中发挥的重要作用，申请者拟通过实验手段验证miR-9/211/142-3p在血睾屏障中对Scrib复合体蛋白的表达调节；在此基础上，通过在体外培养的SC（支持细胞）或SC-GC（生殖细胞）共培养中干预miR-9/211/142-3p的表达，检测其对SC上皮通透性及SC-GC粘附力的影响；并拟在精子释放异常动物模型中，结合体内BTB完整性检测，证实miR-9/211/142-3p可以通过调节Scrib复合体的表达影响生精上皮的周期性进程和精子释放过程，为进一步阐明血睾屏障周期性变化及精子发生的分子机理提供新的思路和实验基础。

In mammals, the periodocal change of the seminiferous epithelium is closely related with the function of BTB and spermatogenesis. In th e preliminary work, the applicant found that miR-9/211/142-3p had seminiferous cycle- dependent expressions in rat testis, the target genes of which were predicted to be Scrib polarity complex by bioinformatics softwares. Due to the important role of Scrib complex in regulation of epithelial function, the expression regulation of Scrib complex by miR-9/211/142-3p will be tested by biological techniques. After that, the Sertoli cell(SC) epithelial permeability and the adhesion between SC and germ cell(GC) will be examined after intervening the expression of miR-9/211/142-3p in cultured SC or SC-GC co-cultured in vitro. Furthermore, the abnormal-spermiation amimal model and BTB integrity assay in vivo will be used to prove the role of miR-9/211/142-3p in seminiferous epithelial cycle and spermiation by regulating the expression of Scrib complex. This study will provide novel thoughts and experimental basis for further clarifying the molecular mechanism(s) of BTB dynamics and spermatogenesis.