Paxillin激活、高尔基体再定位、细胞极化是肿瘤细胞定向运动中重要的调控机制。申请人的最新研究显示，胃泌素通过CCK2R-Ga12/13-RhoA-ROCK途径诱导胰腺癌细胞Paxillin活化，进而促进细胞微丝极化、高尔基体再定位，最终诱导细胞再定向运动。然而，paxillin是通过何种信号途径分别诱导微丝极化、高尔基体再定位、细胞再定向运动的机制目前仍不明确。本项目拟应用细胞分子生物学技术、表达谱测序技术筛查paxillin下游与高尔基体再定位、细胞再定向运动密切相关的差异基因，深入探讨Ga12/13-RhoA-ROCK-paxillin、高尔基体在肿瘤细胞再定向运动中的作用。同时研究将筛查paxillin下游与高尔基体密切相关的差异基因，以及对胰腺癌细胞再定向运动和转移的调控机制。本研究将有助于明确胰腺癌转移的发生机制，并为寻求新的胰腺癌治疗方案提供理论依据。

The phosphorylation of paxillin and relocation of Golgi apparatus and polarization of microtubules cytoskeleton (MTs) are essential for cell directional migration of cancer. Our several studies have confirmed that gastrin promotes the directional migration and reorientation of Golgi apparatus in cancer cells by induction the activation of paxillin via CCK2R/ Ga12/13-RhoA-ROCK signaling pathway, but the detail mechanism is still not clear. The present study was undertaken to investigate the potential downstream cellular signaling pathway and differentially expressed genes of paxillin in regulating Golgi relocation and cell orientated motility in pancreatic cancer cells. The present study contributes to determine the mechanisms of metastasis, and provide theoretical basis for seeking new therapeutic strategy for pancreatic cancer.