基于转录组和蛋白组比较分析的凡纳滨对虾耐寒分子机理研究

凡纳滨对虾是我国水产养殖主导品种，但其不耐寒性已成为制约产业发展的重要因素之一。研究表明，耐寒性状是微效多基因控制的数量性状，基于高通量测序的低温转录组分析是揭示耐寒分子调控机理的有效途径，而蛋白质组是转录组的有效验证和补充，借助两个组学的数据结果，可以验证mRNA和蛋白表达趋势相同的性状相关基因，排除假阳性；发现mRNA和蛋白表达趋势相反或仅蛋白或RNA差异表达的基因及相关通路、揭示基因的转录后调控途径；从而揭示机体性状相关的基因表达调控系统。本研究拟以本团队选育获得的耐寒和不耐寒家系对虾为研究对象，对常温、低温锻炼及脱锻炼对虾分别进行转录组和蛋白组测序，找到耐寒和不耐寒对虾在常温、低温锻炼及脱锻炼时差异表达mRNA和蛋白，进行差异表达mRNA和蛋白的比较关联分析，初步解析耐寒性状相关的分子代谢途径和机理，为查找主效基因和开展分子辅助耐寒品种的选育提供参考。

Litopenaeus vannamei is the dominant farm species for aquaculture in China. However, Litopenaeus vannmei was tropical or subtropical originated species, cold intolerance has become an important limiting factor in the development of the industry. Studies have shown that cold tolerance is a quantitative trait controlled by multiple genes, and low temperature transcriptome analysis which based on high-throughput sequencing is an effective way to reveal the molecular mechanism of cold tolerance. Proteome analysis is an effective verification and supplement of transcriptome.By comparing transcriptomics and proteomics data,differentially expressed genes with the same mRNA and protein expression trend can be vertified,and post-transcriptional gene regulatory pathways can be revealed by analysing genes with different mRNA and protein expression trend. So, the traits related gene expression and regulatory system can be initially revealed by comparative transcriptome and proteome analyses. In the present study, normal temperature,cold acclimation and deacclimation shrimps from both the cold tolerant and cold intolerant family were used for transcriptome and proteome analysis,to find differentially expressed mRNA and proteins.Correlation analysis is to be carried out between differentially expressed mRNA and proteins,and cold associated metabolic pathways and mechanisms will be analyzed, to provide a reference for finding major genes of cold tolerance and carrying out molecular assisted breeding of cold tolerant varieties .