创面炎症与再生的精细调控被认为是再生愈合的核心，IL-10介导的炎症反应与免疫平衡是胎儿无瘢痕再生愈合的关键,创面巨噬细胞是创面炎症反应的关键细胞，IL-10、骨髓间充质干细胞（MSCs）均调控巨噬细胞表型与功能而控制炎症，促进创面修复，但难以实现生理再生愈合。人羊膜间充质干细胞（hAMSCs）具有抗炎、免疫调节和旁分泌等功能。本研究将以hAMSCs为载体进行IL-10基因修饰，运用流式细胞术、免疫荧光和免疫组化、共培养、ELISA法、定量PCR、蛋白印记、小动物活体成像等技术，观察创面局部持续高表达IL-10，协同hAMSCs的功能活性，对创面巨噬细胞表型与功能调控、炎症与免疫及皮肤创面再生愈合的影响，明确IL-10基因修饰hAMSCS对创面修复治疗效果的同时，be巨噬细胞表型与功能及有关炎症免疫调控的变化，为以MSCs为载体的基因治疗用于皮肤损伤，促进再生愈合提供重要依据和新的靶标。

Fine adjustment of inflammation and regeneration are regarded as a core in regenerative healing. It is a key of regenerative healing on no scar in fetal, which inflammatory response and immunologic balance is mediated by IL-10. Wound macrophage is a key cell of inflammatory response. IL-10 and bone marrow mesenchymal stem cell can control by regulation of macrophage phenotype and function，then to promote wound repair. But it is difficulty to realize physiological regeneration healing. Human amniotic mesenchymal stem cells have antiflammatory, immunoregulation and paracrine function and so on. We will decorate hAMSCs as a IL-10 gene expressing victor. These technologies will be used such as flow cytometry, immunofluorescence and immunohistochemical staining, co-culture, ELISA methods, real-time PCR, Western blot and small animal in vivo imaging. The effects will investigate that continue IL-10 high expressing cooperate with hAMSCs activity treat with skin wound on regulation of macrophage phenotype and function, inflammation and immune，regeneration healing. The therapeutic effect of IL-10 gene modificatory hAMSCs will be clarified on wound healing, while the change of macrophage phenotype and function, and regulation of inflammatory and immue will be explained. The about results will apply some important basis and new target on promoting the regeneration of healing of skin.