离体灌注心脏可移植性多尺度关联的无创检测与诊断

心脏移植是治疗心衰末期的“黄金标准”，但供心短缺严重限制心脏移植数量。传统静态冷保存，易造成细胞低温损伤和乳酸中毒，并且无法检测供心可移植性，导致大量可移植供心被废弃。作为新一代供心保存方法，生理温度下机械灌注为供心提供氧气和营养维持其正常生理活动与跳动为供心检测提供了平台。本团队研制了全自动供心离体灌注系统，并成功进行了大量动物试验和三例人心试验。针对临床上对供心可移植性无创检测的迫切需求，本项目研究微观细胞生理特征与宏观心脏功能参数之间的多尺度耦合关联机制，通过深度学习提取隐含在供心各项指标参数中的心脏特征，建立供心移植预测模型。研究基于多传感器信息融合的离体心脏无创精准诊断机理，从而形成一种基于多尺度耦合关联的供心可移植性无创检测诊断新方法，帮助临床医生挑选合格供心。研究成果将为体外心脏的高质量保存与检测提供理论和技术支持，提高心衰末期病人生存率，具有重要的临床和社会意义。

Heart transplantation is the “gold standard” for end-stage heart failure patients. However, donor organ shortage limits the number of heart transplantation. Cold storage is the standard organ preservation method that puts a donor heart into 0 - 4℃ preservation solution until transplantation. It induces a severe decline in organ quality due to anaerobic metabolism and lacks heart functional assessment, resulting in many transplantable donor hearts being discarded. As a novel technology, ex situ heart perfusion maintains the donor heart in a warm beating state and provide an opportunity for heart viability assessment. We have developed a fully automated ex situ heart perfusion instrument and performed over 200 large animals (pig) and three human hearts experiments using our system. In this project, we will develop a non-invasive heart function assessment technique to enable physicians to quantitatively select viable donor hearts. We will study the multi-scale coupling mechanism between physiological characteristics in cell level and cardiac function in large scale level. Through deep learning, a heart transplantation prediction model will be built by extracting heart features from measured parameters. A non-invasive heart function assessment technique will be developed based on multi-sensor information fusion to help physicians to evaluate heart ‘usability’ for transplantation. The research results will provide theoretical and technical support for the high-quality preservation and assessment of donor heart. Ex situ heart perfusion system with non-invasive functional assessment will increase in the number of organs available and reduce the transplantation risk. More qualified donor hearts will help save more lives and families.