卵巢过度刺激综合征（OHSS）是辅助生殖技术促排卵过程中的常见并发症，各种防治措施效果不一，寻求普遍适用的新方法并阐明机制是生殖医学领域的一个热点。我们前期发现电针可防治OHSS，作用的主要效应器官为卵巢，但发挥效应的具体分子、效应物发挥作用的机制未知。我们应用蛋白质组学技术筛选发现CD200在电针后卵巢组织中差异表达显著，提出CD200在电针防治OHSS过程中可能具有特定功能的假说。为此，本项目拟（1）通过临床研究观察电针调控CD200表达与OHSS表型的关系；（2）通过动物实验检测电针后CD200及其受体水平、下游通路关键分子以及血管通透性指标的变化；（3）建立卵巢颗粒细胞和血管内皮细胞条件培养体系，揭示CD200对卵巢颗粒细胞及内皮细胞功能的影响及内在机制。本研究对阐明电针防治OHSS的效应靶标及分子机制、将祖国医学电针疗法引入现代人类辅助生殖技术中具有特别重要的意义。

Ovarian hyperstimulation syndrome (OHSS) is a common complication in ART, it has become research hotspots to explore a new method and elucidate its internal mechanism for preventing the occurrence of OHSS. In our previous study, we found that electroacupuncture (EA) could prevent the development of OHSS, and the main effector is the ovary. However, we do not know the specific molecules and the mechanism of the effector. Then we found out that the ovarian expression of CD200 was significant in proteomic profiling, suggesting that CD200 may have a specific physiology function in ovary in the process of EA on OHSS. Accordingly, this project intends to observe the relationship between the regulation of CD200 expression by electroacupuncture and OHSS phenotype through clinical studies. We plan to detect the changes of CD200 and its receptor levels, downstream pathway key molecules and vascular permeability indexes after electroacupuncture through animal experiments. Finally, we attempt to establish a conditional culture system of ovarian granulosa cells and vascular endothelial cells to reveal the effect of CD200 on the function of ovarian granulosa cells and endothelial cells and the underlying mechanism. The findings are of particular significance to elucidate the effective targets and molecular mechanisms of electroacupuncture in the prevention and treatment of OHSS and to introduce Chinese medical electroacupuncture therapy into modern human assisted reproductive technology.