同源异型框蛋白Msx1和Msx2在中枢神经系统发育中起着重要的作用，其异常表达造成神经系统疾病。申请人前期研究表明，在中枢神经系统特异性地敲除Msx1和Msx2后，小鼠在前脑皮层和小脑部分出现了相对严重的缺陷。但是，Msx1/2在中枢神经系统发育中发挥作用的分子机制还不清楚。解析Msx1/2在中枢神经系统发育中作用机制将有助于我们了解神经系统疾病发生发展的机理。基于申请人未发表的前期研究数据，申请人拟构建Msx1和Msx2敲除的小鼠模型和干细胞系，通过下列实验系统地解析Msx1/2在中枢神经系统中发挥作用的分子机制。1）解析Msx1/2在中枢神经系统发育中的具体作用；2）揭示Msx1/2调控中枢神经系统发育的分子基础；3）阐明Msx1/2在中枢神经系统中发挥作用的分子机制。研究结果将帮助人们了解Msx1/2调控中枢神系统经发育的精细分子作用机制，并为神经系统疾病诊疗提供坚实的理论基础。

The homeoproteins Msx1 and Msx2 play an important role in the development of the central nervous system, and their abnormal expression causes nervous system diseases. Previous studies of the applicant showed that after the specific knockout of Msx1 and Msx2 in the central nervous system, mice developed relatively severe birth defects in the forebrain cortex and cerebellum. However, the molecular mechanism underlying the role of Msx1/2 in the development of the central nervous system remains unclear. Understanding the role of Msx1/2 in the development of the central nervous system will help us understand the mechanism of the occurrence and development of nervous system diseases. Based on the unpublished previous research data, the applicant intends to construct Msx1 and Msx2 knockout mouse models and stem cell lines, and systematically analyze the molecular mechanism of Msx1/2 in the nervous system through the following experiments. 1) To analyze the specific role of Msx1/2 in the development of the central nervous system; 2) to reveal the molecular basis of Msx1/2 regulating the development of the central nervous system; 3) to elucidate the molecular mechanism of Msx1/2 regulating the development of the central nervous system. The results will help people understand the fine molecular roles of Msx1/2 in regulating the development of central mental system, and provide a solid theoretical basis for the diagnosis and treatment of nervous system diseases.