基于整合分类方法玉米螟种团的物种界定研究及疑难种分类地位厘定

玉米螟种团Ostrinia nubilalis species group隶属于草螟科Crambidae、野螟亚科Pyraustinae，全世界已知10种、23亚种，我国报道10种、11亚种，是一类重要的农业害虫。但是长期以来就该种团物种界定这一核心问题的研究都不够深入，导致很多疑难种的分类地位及种间的系统发育关系甚至分类系统备受质疑和争议，严重制约了分类与害虫防治等研究工作的进展。本项目拟采用多方面证据，综合运用几何形态测量学、DNA条形码技术、线粒体基因组学、分子系统学及谱系生物地理学等方法，对该种团全部10种、23亚种展开研究，旨在整合分类研究和重建该种团的系统发育基础之上，廓清种间的形态学与分子界定依据及标准，对所有疑难种的分类地位进行重新厘订。同时，本研究也将有助于解析该种团的物种起源、分化和形成机制，还可为野螟亚科乃至鳞翅目昆虫众多种团的物种界定和分类研究提供科学依据。

The Ostrinia nubilalis species group is one of the most taxonomically difficult groups in the subfamily Pyraustinae (Lepidoptera: Crambidae), including 10 species and 23 subspecies worldwide. Ten species and 11 subspecies have been reported from China. Many species are serious and important crop pests. However, the species boundaries in this group has been puzzled for a long time ledding to many sibling species are morphologically indistinguishable and accurate species identification is extremely difficult. A number of issues are indispensable to need thorough taxonomic scrutiny and further analysis. On the basis of previous taxonomic works, we attempt to reconsider a comprehensive systematic study on this group combined morphological characters, mitochondrial genome, nuclear genes and geographical distribution. The purpose mainly focuses on three aspects. 1. Reconstruction the phylogenetic relationship within the species group. 2. A taxonomic revision on the species group worldwide. 3. Propose the reliable morphological and molecular species delimitation boundary . This study will be great helpful to advance our understanding of speciation mechanisms of this species group and to support further work on many species group within the subfamily Pyraustinae and even for other group in Lepidoptera.