应激颗粒对新城疫病毒复制与先天性免疫的调控机制研究

新城疫病毒（NDV）是危害全球养禽业最主要的病原之一，也是一种特异性的溶瘤病毒，应激颗粒（SG）在宿主细胞抵御病毒感染时发挥重要的作用，为了探明SG在NDV感染宿主细胞过程中有何作用，以及NDV感染后SG对先天性免疫通路的调控机制，本项目拟进行以下研究：（1）以NDV感染HeLa和DF-1细胞后，检测NDV通过何种通路诱导SG形成；（2）通过病毒蛋白和SG相关蛋白互作实验结合感染性克隆检测参与SG形成的主要病毒蛋白；（3）以RNA干扰等手段对SG进行阻断后，检测NDV的复制水平以及先天性免疫通路激活情况。研究结果对揭示SG对NDV复制与先天性免疫的调控机制具有重要意义，将增进NDV与宿主细胞互作的认识，有利于发现新的抗病毒靶点，为更安全有效的肿瘤治疗奠定理论基础，另一方面，也为其他病毒感染细胞后SG在先天性免疫中的调控作用提供借鉴。

Newcastle disease virus (NDV) is one of the most important pathogens which do a great harm to world poultry industry. Moreover, NDV is a specific oncolytic virus. Stress granule (SG) plays an important role in resisting virus infection. To explore the role of SG in NDV infection and regulation mechanisms of innate immunity pathway, this project conducts the following studies. (1) HeLa and DF-1 cells are infected with NDV and stained with SG specific marker to test SG formation. The pathway involved in SG formation induced by NDV infection is then identified. (2) The main virus structure proteins involved in SG formation is investigated by the interaction assay between virus protein and SG associated protein. Reverse genetics system is used for further identification. (3) The replication ability of NDV and RIG-I mediated IFN-I signaling pathway are tested after blockage of SG by siRNA or pharmacological treatments. These results reveal the mechanism of how SG modulates NDV infection and innate immunity. This project will enhance the knowledge of NDV-cell interaction, benefiting to discovery of new antiviral target and tumor treatment in a safe and efficient way. In another way, this project also offers insight into how SG modulate innate immunity after infection of other viruses.