miR-155/Jarid2基因修饰BMSCs调控溃疡性结肠炎TH17分化机制及复方苦参汤的协同作用

肠黏膜免疫紊乱是导致溃疡性结肠炎（UC）发生、发展和影响其转归的决定因素，Th17是UC关键促炎因子，Th17细胞及其相关细胞因子在炎症的进程中发挥重要作用。miR-155/Jarid2轴在Th17分化及效应阶段调节细胞因子产生过程中发挥重要作用。骨髓间充质干细胞（BMSCs）作为一种新的治疗方法能治疗炎症介导的多种疾病，干细胞治疗的疗效有待进一步提高，对BMSCs进行基因修饰，是细胞或基因治疗的发展方向。通过对BMSCs进行miR-155/Jarid2基因表达沉默，从而使其定向控制Th17细胞分化，复方苦参汤（清热燥湿凉血方）起到协同作用，控制炎症进程，从而提高BMSCs治疗UC的疗效。本课题通过免疫组化、分子生物学等方法，研究基因沉默miR-155/Jarid2修饰后的BMSCs调控Th17细胞分化治疗UC及复方苦参汤的协同作用，为开拓该病的临床治疗手段提供新的理论依据和研究方法。

Intestinal mucosal immune disorders determines the development of ulcerative colitis(UC) and impacts of its outcome. Th17 is one of a key pro-inflammatory cytokines in IBD, then Th17 cells and its associated cytokines play an important role in the inflammation process. miRNA-155 / Jarid2 axis play an important role in inprogramming both the Th17 differentiation and the effector phase adjustment process to produce cytokines. Bone Marrow-Derived Mesenchymal Stem Cells (BMSCs) as a new method of treatment which can treat a variety of inflammation-mediated diseases, but stem cell therapy efficacy remains to be improved. Genetically modified BMSCs is the development direction of cell therapy or gene therapy. This paper intends to modify BMSCs with miRNA-155 / Jarid2 silence gene expression, making it directional control of Th17 cell differentiation, and Compound sophorae decoction(Qingreliangxue dampness square )synergistic effect, then control inflammation process, thereby enhancing the efficacy of BMSCs treatment of ulcerative colitis.Through immunohistochemical, molecular biology, this topic studies miRNA-155 / Jarid2 gene-modified BMSCs to regulate intestinal Th17 cell differentiation in the treatment of UC and the synergy of compound sophora soup.It will provide new theoretical basis and research methods for clinical treatment of UC.