毛乳头细胞在毛囊生长和周期更替中具有重要意义，其功能异常是导致毛发再生障碍和脱发性疾病的主要因素。申请者前期研究发现链接粘附分子JAM-A基因在人毛囊毛乳头细胞随毛发周期不同呈差异表达，但在过表达研究中发现，JAM-A调控毛乳头细胞的功能与其蛋白无关，而是通过其mRNA的3’UTR发挥作用。经过预测及初步验证，我们推测JAM-A 3'UTR 通过内源性竞争性RNA(ceRNA)机制影响多功能蛋白聚糖的表达，进而影响人毛乳头细胞的功能，影响毛囊再生。为进一步明确该假说，本课题拟构建不同区段JAM-A mRNA过表达及敲除的人毛乳头细胞株，对比分析JAM-A CDS区和3’UTR区对人毛乳头细胞生物学功能的影响。并进一步通过C3H/HeJ小鼠斑秃模型，探讨JAM-A 3’UTR体内干预对毛囊周期及再生的影响，明确该基因在毛发再生中的作用，并为相关疾病的研究提供新的靶点。

Dermal papilla cells (DPC) play an important role in the growth cycle of hair follicles and hair regeneration. The biological function abnormality of DPC is the primary factor for hair regeneration obstacle and alopecia. In the previous study,applicant discovered junction adhesion molecule A (JAM-A) gene was differentially expressed in human DPC in various hair growth cycle. But in the overexpression study, the results show that the regulation of DPC biological function independent JAM-A protein encoding region, instead, via 3’Untranslated Region of JAM-A mRNA. After bioinformatics prediction and preliminary validation, we speculate that JAM-A 3’UTR 'effect on the expression of versican by ceRNA mechanism, thereby affecting the activity and biological function of human DPC, affect hair regeneration. In order to further clarify the hypothesis, we intend to build human DPC line with JAM-A over expression and knockdown, comparative analysis of the effect of JAM-A CDS region and 3’UTR region intervention on human DPC biological function. And further through C3H/HeJ alopecia areata mice model to investigate the effect of JAM-A 3’UTR in vivo intervention on hair cycle and hair regeneration, clear the gene’s role in human hair regeneration, and also, provide a new target for the research hair loss related diseases.