肌硬膜桥对脑脊液循环的动力学作用的研究

肌硬膜桥是新近发现的位于人枕下区肌和硬脊膜之间的纤维连接。本课题组在国际上率先提出肌硬膜桥为脑脊液动力来源之一的原创性理论，发现肌硬膜桥可能与部分原发性弥漫性头痛有关；进一步丰富了肌硬膜桥的空间结构、纤维构成，率先发现此结构普遍存在于哺乳纲、爬行纲及鸟纲，在演化上具有高度的保守性。肌硬膜桥主要由I型胶原蛋白构成，博来霉素对I型胶原蛋白具有显著的增生作用；Pax1基因敲除鼠将发生椎骨形态及椎管体积的改变，继而引发肌硬膜桥形态变化。肌硬膜桥的I型胶原蛋白增生或形态变化都会对脑脊液动力循环产生影响。本研究利用Pax1基因敲除及博来霉素诱导肌硬膜桥胶原纤维增生小鼠模型，采用组织学，扫描电镜，透射电镜技术，微创压力传感仪，MR及小动物活体荧光成像系统监测肌硬膜桥改变引发脑脊液循环变化的各项指标。肌硬膜桥对脑脊液循环的动力学作用效果的进一步明确，将为部分原发性头痛等神经系统疾病的诊疗提供重要的指导。

Myodural bridge (MDB) is a newly discovered fibrous connective tissue between the muscle and the spinal dura mater in the suboccipital region of the human body. Our research group proposed an original theory that the Myodural bridge is one of the dynamic sources for cerebrospinal fluid (CSF). Also it was found that MDB may be related to some primary diffuse headache. MDB’s researches were further enriched in the spatial structure and fiber composition in our group. It was even first discovered that MDB is a universal existing structure in mammals, Reptilia and Aves. So it may be a highly evolutionarily conservative structure. The MDB is mainly composed of Collagen Type I, and bleomycin has a significant proliferation effect on Collagen Type I. Vertebrae morphology and spinal canal volume would change in Pax1 gene knockout mice, and these changes may lead to morphology changes of MDB. Collagen Type I proliferation or morphological changes in MDB can affect the dynamic circulation of CSF. In this study, Pax1 gene knockout mice and of collagen fibers proliferation of MDB mice were used. Histology, scanning electron microscopy, transmission electron microscopy, minimally invasive pressure sensor, MR and fluorescence imaging system of live animals were used to monitor various changes indicators in CSF circulation caused by changed MDB. The further clarification for the dynamic effect of the MDB on CSF circulation will provide an important guidance for the diagnosis and treatment of some primary headache and other neurological diseases.