细胞蛇是最新发现的一种细胞器，大多由代谢酶组装形成“蛇”形结构，第一个已知成分是CTP合成酶（CTP synthase，CTPS）。目前关于细胞蛇功能的研究尚处于起步阶段，CTPS细胞蛇在骨骼肌卫星细胞增殖分化过程中作用仍不清楚。本研究拟以小鼠骨骼肌卫星细胞C2C12为模型，首先筛选CTPS细胞蛇组装的关键氨基酸残基，再将CTPS基因过表达并形成细胞蛇的C2C12细胞进行转录组和蛋白质组测序，结合生物化学中CTP合成过程，挖掘参与CTPS细胞蛇组装的关键基因和蛋白因子，初步探索C2C12细胞中CTPS细胞蛇组装的分子机制。在此基础上，研究CTPS蛋白及其形成的细胞蛇对C2C12细胞增殖、分化和凋亡的影响。最后检测小鼠肌肉损伤修复过程中CTPS细胞蛇的形态和数量，探明CTPS细胞蛇在C2C12细胞增殖和分化中的作用及机制，为明确CTPS细胞蛇在肌肉干细胞命运决定中的作用提供初步的实验依据。

Cytoophidia are newly discovered organelles, which are filaments and compartmentation of metabolic enzymes. The first known metabolic enzyme is CTP synthetase (CTPS). At present, the research on the function of cytoophidia is still in its infancy, and the roles of CTPS cytoophidia in the proliferation and differentiation of skeletal muscle satellite cells are still unclear. In present study, the C2C12 cells will be used to explore the roles and mechanisms of CTPS on the proliferation and differentiation in skeletal muscle satellite cells. At first, the key amino acid residues of CTPS will be screened. And then, RNA-Seq and iTRAQ will be performed to screen the key genes and protein factors that are involved in the cytoophidia assembly in the C2C12. On this basis, the effects of CTPS protein and CTPS cytoophidia on the proliferation, differentiation and apoptosis of C2C12 cells were studied. Finally, we would detect the morphology and number of CTPS cytoophidia in the process of muscle damage repair in mice, to explore the roles and mechanisms of CTPS cytoophidia in C2C12 cell proliferation and differentiation. These would provide a preliminary experimental basis for illuminating the roles of CTPS cytoophidia in determining the fate of muscle stem cells.