寄主昆虫如何识别寄生蜂为"非我"是非常重要的科学问题，但有关其分子机制的研究还非常有限。本项目拟以腰带长体茧蜂和其容性及抑性寄主为研究对象，阐明lectin在鳞翅目寄主识别寄生蜂为"非我"过程中的功能机制，以及它与蜂胚胎表面的保护因子hemomucin和寄主血细胞表面的受体integrin的相互作用。项目首先拟在两个鳞翅目昆虫中鉴定与寄生蜂识别相关的凝集素因子PRRlecs，制备它们的多克隆抗体，检测其分布和表达模式，并表达重组蛋白，分析它们的糖结合特异性及对寄主血细胞包囊寄生蜂的影响，以期证明它们是重要的识别寄生蜂的因子；然后通过比较容性寄主与抑性寄主中PRR-lectins的分布及表达差异，分析腰带长体茧蜂在不同寄主中命运不同的原因；最后探究凝集素的免疫功能是否与调控血细胞粘附及包囊的膜受体integirn有关。为阐明鳞翅目昆虫识别近缘寄生物的分子机制打下基础。

How do host insects recognize the parasitoid as non-self is an important scientific question,however the molecular mechanisms about it are still unclear until now. The proposal plans to understand the mechanisms that how do lectins in Lepidoptera host larvae recognize parasitoid as non-self and the relationships between lectin and hemomucin, a factors protect M.cingulum to avoid the encapsulation of host's hemocytes, and integrin, a receptor related to hemcoyte's spreading and encapsulation. The animal materials used in the proposal are the parasitoid M. cingulum and its capacitive host Ostrinia furnacalis and suppressive host Spodoptera exigua. At first, Parasitoid Recognition Related lectins, PRR-lecs will be identified and cloned in two Lepidoptera, and polyclonal antibodies against PRR-lecs will be prepared to locate lectins in tissures and hemocytes and analyze their expression pattern. Reconstructed PRRlecs(rPRR-lecs) will be expressed and purified to analyze the specific sugar-binding characters of PRR-lecs and effects on spreading of host hemocytes and encapsulation of parasitoid embryos. All studies above will clarify that PRR-lecs are important parasitoid recognition related lectins. Furthermore, the differences of PRRLecs in two Lepidoptera will be compared to clarify the reason that how M. cinglum may avoid the immune reaction of capacitive host O. furnacalis. Finally, if the immune function of lectins are related to the receptor-integrin on hemocytes is studies. All results will lay the fundation for clarifing the molecular mechanisms that how do Lepidoptera recognize related parasitoid as non-self.