拮抗章鱼胺受体阻断产卵的害虫防治技术研究

害虫超强的繁殖能力是其爆发成灾，持续危害农作物并迅速发展抗药性的主要原因。人们很早就希望通过干扰害虫的生殖从而把种群限制在可控的范围内，但是一直缺乏简单有效的方法和安全特异的作用靶标。产卵是昆虫繁殖行为过程中的关键一步，章鱼胺和其对应受体起到关键的调控作用，遗传学研究表明缺乏章鱼胺受体OA1或OA2B2其中的任何一个都会导致雌虫不育。因为章鱼胺受体只存在于无脊椎动物，并具有靶标成药性，基于我们前期在水稻害虫章鱼胺受体上的分子药理学和生理学研究，本项目提出构建表达褐飞虱OA1和OA2B2受体的细胞系并进行高通量筛选，找出高效特异的章鱼胺受体的小分子拮抗剂，测试其阻断害虫产卵行为的效果。这样通过小分子化合物抑制雌虫产卵而不影响交配和雄虫，不仅可以大幅降低种群数量达到防治效果，还可以缓解自身和其他杀虫剂抗性的发展，提供了一种全新的基于行为调控的害虫防治策略和手段。

The superb reproductive capacity of pests is the main reason for their outbreaks, continuous damage to crops and rapid development of insecticide resistance. People have long hoped to limit the population to a controllable range by interfering with the reproduction of pests, but there has been a lack of simple and effective methods as well as safety and specific targets. Egg-laying is the key step in the process of insect reproduction in which octopamine and its corresponding receptors play a key regulatory role. Genetic studies have shown that the lack of any of the octopamine receptors OA1 or OA2B2 can cause female infertility. Since octopamine receptors only exist in invertebrates and are druggable, according to our previous molecular pharmacology and physiology studies on the rice pest octopamine receptors, this project proposes to construct cells expressing brown planthopper OA1 and OA2B2 receptors and perform high-throughput screening to find highly effective and specific small molecule antagonists of octopamine receptors, and further tested their effectiveness in blocking the egg-laying behavior of rice pests. This method through inhibiting the egg-laying of females by small molecule compounds will not affect mating and males, can greatly reduced pest populations and inhibit the development of resistance to itself and other insecticides. It provides a new behavior-based regulation control strategies.