大河乌猪具有肌内脂肪含量高的种质特性，是制作云南高原特色畜产品—宣威火腿的原料。大河乌猪肌内脂肪含量高的种质特性必然与其肠道微生物密切相关。本项目拟以大河乌猪为试验材料，以长白猪为对照，首先测定背最长肌肌内脂肪含量，利用HPGC检测回肠、空肠和盲肠各肠段短链脂肪酸含量，采用16S rRNA和宏基因组学测序比较大河乌猪与长白猪肠道微生物群落及基因功能差异，关联分析微生物区系-代谢物及肌内脂肪含量，筛选可能与肌内脂肪含量相关的微生物菌群及优势群落基因；利用real time PCR技术定量检测差异微生物群落基因丰度，从而阐明猪肠道微生物区系-代谢物及宿主肌内脂肪沉积间相互调控机制，该研究结果将丰富乌金猪肌内脂肪沉积的分子机制理论，为深度挖掘和高效利用乌金猪优良种质资源，开发安全、健康的畜产品提供理论依据，为通过营养措施调控肠道微生物，增加猪肌内脂肪沉积，改善猪肉品质，提供新的思路和可行的途径。

Wujin pigs as Yunnan excellent local breeds with special characteristics of higher intramuscular fat content, is the raw material to produce Xuanwei ham. The traits of higher intramuscular fat content in Wujin pigs are closely related to the special intestinal microflora. On the basis of our previous studies and combined with the domestic and foreign research focus, The project intends to use Wujin pigs as experimental materials and Landrace as control. Intramuscular fat content was determined in Longissimus dorsi muscle. Fatty acid concentrate in different intestinal segment will be detected firstly by high pressure liquid chromatography (HPGC). Microbial flora diversity will be measured using 16S rRNA and metagenomic analysis technology. The different intestinal microflora between Wujin and Landrace pigs would be analyzed and to screen the microbial flora which may be associated with the content of metabolites and intramuscular fat. The abundance of microbial communities would be determined by real time PCR technology. The results of this study will enrich the molecular mechanism theory of intramuscular fat deposition in Wujin pig and provide the theoretical basis to mine and use Wujin pigs resources, to develop the safe, health of Yunnan plateau characteristics of animal products. The study would provide new ideas and feasible ways through nutrition regulation and control measures of gut microbes to increase porcine intramuscular fat deposition, improve meat quality.