自身免疫异常与系统性硬化病（SSc）的发生发展密切相关。Th17/Treg细胞失衡在SSc自身免疫功能紊乱中起着重要作用，是治疗SSc自身免疫异常的关键靶点。前期研究表明，温阳化浊通络方治疗 SSc临床效果显著，并能改善SSc患者体内Th17/Treg细胞失衡，增加外周血中FOXP3的mRNA表达水平，降低RORC、DNMT1/3A的mRNA表达水平。本项目拟从温阳化浊通络方通过影响RORC/FOXP3 DNA甲基化修饰从而调控Th17/Treg细胞平衡的角度出发，在体外淋巴细胞水平和SSc小鼠模型的整体水平上考察温阳化浊通络方对RORC/FOXP3甲基化修饰及Th17/Treg细胞分化、增殖、免疫激活/抑制功能等的影响，明确其改善SSc Th17/Treg细胞失衡的作用机制，探讨温阳化浊通络方通过改善Th17/Treg细胞失衡而发挥抗自身免疫反应的作用机制，为SSc的防治提供科学依据。

Autoimmune abnormality is closely related to the occurrence and development of SSc. Th17/Treg cell imbalance plays an important role in SSc autoimmune disorders and is a key target in the treatment of SSc autoimmune abnormalities. Our previous study indicated that Wenyang Huazhuo Tongluo Recipe (WYHZTLR) based on lung, spleen and kidney-skin correlation theory treated effectively SSc patients, which can improve the imbalance of Th17/Treg cells, and furthermore increase the mRNA expression level of FOXP3 in SSc patients peripheral blood and decrease the mRNA expression level of RORC,DNMT1/3A. The aim of this project was to investigate the effect of WYHZTLR on the balance of Th17/Treg cells via regulating RORC/FOXP3 DNA methylation. At the lymphocyte level and animal model of SSc level we will study the effects of WYHZTLR on the methylation of RORC/FOXP3 and the differentiation, proliferation, immune activation/inhibition of Th17/Treg cells, in order to reveal the meachnism of WYHZTLR improve imbalance of Th17/Treg cells. The systemic studies of anti-autoimmune response by WYHZTLR via improving the Th17/Treg cell imbalance can provide scientific basis for the treatment of SSc.