七星瓢虫非昆虫源人工饲料配方优化及其质量评价

瓢虫人工饲料的研究始于20世纪50年代，到目前为止仍然没有一种理想的、可以规模化繁殖瓢虫的人工饲料。研究昆虫人工饲料的困难在于对调控一种昆虫种群适合性的生理生化过程缺乏了解，且缺少能用来评价昆虫对特殊营养源适合性的生理或生化标记。通过基因组测序及转录组分析，了解含不同营养因子的人工饲料对昆虫基因表达模式的影响，从而确定不同营养因子对昆虫营养和生理生化的影响，可为昆虫人工饲料研制提供一种新方法。本项目拟通过正交试验法分别筛选七星瓢虫幼虫和成虫的人工饲料配方，然后将取食不同饲料配方的七星瓢虫幼虫和成虫进行转录组测序分析差异表达基因与营养差异的相关性，进一步优化七星瓢虫幼虫和成虫饲料配方，比较七星瓢虫幼虫和成虫人工饲料营养成分差异，同时对优化饲料进行七星瓢虫饲养效果评价和捕食功能测定。以期揭示七星瓢虫成虫和幼虫最适营养需求，明确优化饲料对七星瓢虫生物指标和捕食能力的影响。

Research on artificial diets of ladybugs began in the 1950s. However, no ideal artificial diet for large-scale ladybug breeding has been developed. The difficulty in studying insect artificial diets is the lack of physiological or biochemical markers that can be used to evaluate the suitability of insects to specific nutrient sources. It could be a new method for the development of artificial diets for insects to determine the effects of different nutrient factors in artificial diets on the gene expression patterns of insects through transcriptional sequencing group analysis. This research intends to screen the artificial diet formulas of Coccinella septempunctata larvae and adults using orthogonal tests. Furthermore, the genomes of the larvae and adults fed the different diet formulas were sequenced to analyze the correlation between differential gene expression and nutritional ingredient variance and to further optimize artificial formulas for larvae and adults. The formulas for larvae and adults were also compared to evaluate their respective feeding effects and predation of Aphis craccivora. The purpose of this research was to reveal the optimal nutritional requirements of adult and larvae of the C. septempunctata, and to determine the effect of optimized diet on the biological indexes and predation ability of the C. septempunctata.