补肾助孕方是国医大师夏桂成教授经验方，临床疗效确切，前期研究证实该方可通过调节生殖轴分泌功能，改善子宫内膜容受性，进而矫治LPD性不孕症。进一步研究表明，着床期子宫内膜局部炎症水平异常也是LPD的表现之一，而补肾助孕方可调节着床期子宫内膜炎性因子表达。鉴于炎性因子与氧化应激（OS）产物可通过Keap1-Nrf2-ARE信号转导系统，共同影响子宫内膜局部炎症水平，进而影响子宫内膜容受性，因此，我们提出假说：补肾助孕方可能通过影响着床期子宫内膜OS水平，干预Keap1-Nrf2-ARE信号转导通路，进而改善子宫内膜局部炎性环境和容受性，最终促进胚胎着床。本项目拟选用LPD大鼠模型与OS细胞模型，采用基因沉默、免疫细胞分选、线粒体功能检测等生物学技术，多角度、多层次研究补肾助孕方对子宫内膜Keap1-Nrf2-ARE系统以及OS水平的调控作用，进一步阐明补肾助孕方治疗LPD性不孕的作用机制。

The Prescription for Reinforcing Kidney and Promoting Pregnancy (Bu Shen Zhu Yun Fang, BSZYF) is a rich experienced formula proposed by Professor Xia Guicheng, the “National TCM Master” and has remarkable curative effect. Previous studies have shown that this prescription can improve endometrial receptivity by regulating reproductive axis secretion function to treat LPD infertility effectively. Further studies have proved that abnormal inflammation level in endometrium during implantation is also one of the manifestations of LPD. BSZYF can regulate the expression of inflammatory factors in endometrium during implantation. Based on the fact that inflammatory factors and the products of oxidative stress (OS) can affect the local inflammation level of endometrium through Keap1-Nrf2-ARE signal transduction system to affect endometrial receptivity. Therefore, we are proposing the hypothesis that the BSZYF may affect the OS level of endometrium during implantation and interfere with Keap1-Nrf2-ARE signal transduction pathway to improve the local inflammatory environment and receptivity of endometrium. Then BSZYF can ultimately achieve the goal of promoting embryo implantation.. In this project, we will establish rat LPD model and cell OS model. We will use gene silencing, immune cell sorting, mitochondrial function detection and other biological techniques to study the regulation effect of BSZYF on Keap1-Nrf2-ARE system and OS level in endometrium from multiple perspectives and levels. Thus, we will further clarify the mechanism of BSZYF in the treatment of LPD infertility.