禾谷镰刀菌中RNA结合蛋白FgRbp1调控前体mRNA剪接的机制研究

在我国，由禾谷镰刀菌复合种引起的小麦赤霉病严重威胁小麦生产安全。对病原菌致病与生长发育机理的深入研究将为赤霉病的防治提供理论依据。本项目前期在禾谷镰刀菌中鉴定到一个对病菌致病、生长至关重要，但其功能还未知的RNA结合蛋白FgRbp1。通过互作蛋白筛选、高通量测序发现该蛋白参与到对菌体中前体mRNA剪接的调控。但是，FgRbp1调控剪接的分子机制尚未明确。因此，本项目将进一步鉴定FgRbp1在菌体内结合的靶标mRNA；解析FgRbp1对靶标mRNA的结合与剪接之间的关联；阐明FgRbp1通过与互作蛋白FgU2AF23互作而调控禾谷镰刀菌中前体mRNA剪接的分子机制；并探索沉默FgRbp1来抑制前体mRNA剪接，以提高内含子包含基因的沉默效率，从而为基于RNA干扰的小麦抗赤霉病育种提供新视角。

Fusarium head blight (FHB) caused by Fusarium graminearum species complex is a serious threat to wheat production in China. Further study on the mechanism of pathogenicity and development of the pathogen will provide a theoretical basis for the control of FHB. Our previous study identified an uncharacterizecd RNA binding protein named FgRbp1 in F. graminearum, and found it is critical for the development and pathogenicity of this fungus. By screening the interacting proteins of FgRbp1 and high-throughput sequencing, we found that FgRbp1 is a pre-mRNA splicing regulator. However, the molecular mechanism of FgRbp1 in regulating splicing remains unclear. This project intends to identify the target mRNAs of FgRbp1, analyze the relationship between the FgRbp1’s binding to mRNAs and pre-mRNA splicing and explore the molecular mechanism of the regulation of FgRbp1 in splicing via interacting with FgU2AF23. Furthermore, this project will explore silencing FgRbp1 to inhibit the splicing to improve the silencing efficiency of intron containing genes, which will provide a new perspective for FHB-resistant breeding based on RNA interference.