囊泡转运参与重要膜蛋白功能的调控机制

植物细胞膜蛋白作为细胞膜的重要组分，是许多生物学功能的执行者。由囊泡转运所介导的双向性运输在细胞内外物质交换和信号传递过程中发挥重要作用。然而，囊泡转运是如何调控细胞膜蛋白的功能，在植物中尚缺乏深入的研究。针对前人工作的不足，本项目将以拟南芥中两类6个膜蛋白为研究对象，着重开展四方面的研究：(1)分析膜蛋白在质膜上的运动和聚合状态，以及在膜微区内外的分布与运动规律；(2) 比较分析膜蛋白的胞吞方式及胞吞速率；(3) 膜蛋白的胞内转运途径和蛋白去向的研究；(4) 不同处理后，膜蛋白的运动特性、胞吞方式和胞内转运变化规律的研究。在本项目中，拟采用可变角度的全内反射荧光显微术、荧光相关光谱、荧光互相关光谱等新技术。无疑，本研究结果将为阐释植物细胞中囊泡转运参与膜蛋白功能的调控机制奠定基础。

As main components of plasma membrane in plant cells, membrane proteins play a major role in several biological processes. It is well demonstrated that the biphasic transport mediated by vesicle trafficking is crucial for macromolecule transportation and signaling between the cells and their external environment. However, the mechanism of how vesicle trafficking regulates membrane proteins to maintain their homeostasis in plants remains to be further elucidated. In consideration of the insufficiency of previous investigations, this project mainly focuses on the endocytic pathways and intracellular trafficking routes by means of dynamic analysis in the living cells together with genetic approaches in 6 different membrane proteins. First, we will determine the distribution patterns and dynamic properties of membrane proteins, as well as their oligomerization states in different membrane microdomains. Then we will compare the endocytic pathways and endocytic velocities in 6 different membrane proteins. Moreover, we are going to investigate the intracellular trafficking and final destination of membrane proteins. Finally, we will further explore the variations in dynamic properties, endocytic pathways and intracellular trafficking routes of membrane proteins in response to different treatments. In this investigation, some new techniques including variable-angle total internal reflection fluorescence microscopy, fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy will be used. Surely, the results of this investigation will lay foundation for the better understanding of the regulatory mechanisms of vesicle trafficking involved in the functional characterization of membrane proteins in plants.