IL-27属IL-12家族细胞因子，主要作用于固有免疫系统和获得性免疫系统的各种细胞而发挥免疫调节作用，但具体机制不明。我们的前期研究发现银屑病患者外周血单一核细胞（PBMC）和皮损组织中IL-27P28和IL-27EBI3mRNA表达水平下调，IL-17A、IL-21、IL-22mRNA表达水平上调，银屑病PBMC中Th17细胞比例明显升高。鉴于IL-27在银屑病的发病中可能发挥的重要作用，本课题主要研究IL-27对银屑病iNKT细胞和Th17细胞的免疫调节和效应应答的作用。分离外周血幼稚CD4+T淋巴细胞，体外培养，使用IL-27刺激，分析IL-27对上述细胞的激活、增殖分化和免疫调节作用；同时建立咪喹莫特诱导的小鼠银屑病样皮损模型，使用IL-27和抗IL-27单抗治疗，研究银屑病样皮损的发展变化，阐明IL-27在银屑病发生过程中的作用，为临床上靶向治疗提供理论依据。

Interleukin-27 (IL-27) belongs to the IL-12 family which plays a wide range of immune regulation effect on various immune cells in innate and adaptive immunity. Our previous studies found that the levels of IL-27P28 and IL-27EBI3 mRNA were significantly decreased in PBMC and skin lesion from patients with psoriasis compared to healthy controls. The levels of IL-17A, IL-21, IL-22mRNA in PBMC and skin lesions from patients with psoriasis were higher than those in healthy controls. Th17 cells in PBMC were significantly increased in patients with psoriasis. In the present study, we will investigate immune regulation of IL-27 on iNKT cells and Th17 cells. We firstly isolate Naive CD4+ T cells from PBMC in patients with psoriasis and co-culture with IL-27 in vitro, then evaluate the impact of IL-27 on the proliferation and differentiation of iNKT cells and Th17 cells. We will further generate imiquimod-induced psoriasis-like skin lesions model in mice, assess the changes of psoriasis lesions, cytokines and chemokines using rIL-27 or anti-IL-27 antibody by multiple subcutaneous injection or by intraperitoneal injection. This study could suggest the possibility of targeting IL-27 for therapeutic treatment of psoriasis.