基于Th1免疫应答增强探讨穴位贴敷诱发接触性皮炎对哮喘气道炎症的反向调节机制

穴位贴敷在预防和减轻哮喘反复发作中疗效确切。多数患者在贴敷部位出现接触性皮炎改变，皮炎的发生与疗效呈正向关系。但穴位贴敷对哮喘气道炎症改善的作用机制不明，且贴敷结束后对气道炎症改善的维持时间仍不清楚。诱发接触性皮炎可使皮肤中Th1免疫应答增强，通过全身免疫应答导致气道Th2免疫应答抑制，保持Th1/Th2免疫平衡，从而减轻气道炎症。前期研究发现：穴位贴敷可改善哮喘气道炎症，其诱发的接触性皮炎存在Th1免疫应答增强现象。本研究拟在前期研究的基础上，建立慢性哮喘小鼠模型，穴位贴敷诱发其接触性皮炎。从Th1/Th2免疫平衡中Th1免疫应答增强层面，研究穴位贴敷诱发接触性皮炎对气道炎症影响的作用机制。从穴位贴敷结束后血中Th1/Th2比值动态变化层面，明确穴位贴敷改善气道炎症的维持时间。进而从穴位贴敷诱发接触性皮炎以气道炎症的免疫调节角度部分阐释“肺外合皮毛”的科学内涵。

Point application is an effective treatment to prevent and reduce the recurrence of asthma. Most of the patients had contact dermatitis on the pasting site after point application. The occurrence of contact dermatitis had positive correlation with clinical efficacy. The mechanism has been unknown till now that contact dermatitis induced by point application can improve the airway inflammation in asthma. Moreover, how long the effect maintains is unclear after the point application. It is believed that inducing contact dermatitis can cause the skin Th1 immunoenhancement and the airway Th2 immunosupression through systemic immune response. And the immune balance of Th1/Th2 is kept which causes the improving of airway inflammation in asthma. It was found in the previous studies that point application could improve airway inflammation in asthma. And Th1 immunoenhancement was found in contact dermatitis induced by point application in asthma.On the basis of previous studies, we are going to establish mice model with chronic asthma, and to induce its contact dermatitis by point application. From the aspect that turning Th1/Th2 immune balance to Th1 immunoenhancement, we tend to study the mechanism of that contact dermatitis caused by point application could improve airway inflammation. From the aspect that the dynamic changes of Th1/Th2 ratio in the blood after point application, we tend to observe the lasting time of improving airway inflammation induced by point application. In addition, we tend to clarify the close relation between lung and skin according to immune regulation mechanism between contact dermatitis caused by point application and airway inflammation.