ERM对桃叶杜鹃抗旱性的调控机制

以优良抗旱杜鹃花类菌根（ERM）真菌菌株接种桃叶杜鹃（不接种对照），进行水分胁迫试验，从细胞与生理代谢水平两个层次对ERM调控桃叶杜鹃抗旱生理机制进行研究。在细胞水平上，通过对桃叶杜鹃菌根苗的菌根显微结构、植物细胞精细结构分析，研究共生根系及植物细胞抗旱或御旱的形态变化；在生理代谢水平上，对接种苗重要内源植物激素、光合特性特别是光合产物的积累与分布、渗透调节以及相关酶活性等多个方面的测试与相关关系分析，探讨桃叶杜鹃菌根苗抗旱生理机理。项目验证科学假设：菌根化桃叶杜鹃抗旱性的增加是因为ERM迅速地积累、促进或改变植物激素合成（特别是作为干旱信号的脱落酸ABA）；同时或进而影响或调控光合、抗氧化酶活性等因素，从而调节植物整体的生理生化反应以应对干旱胁迫。项目研究的意义在于为桃叶杜鹃引种驯化、节水栽培与园林应用提供理论依据，同时为高山常绿杜鹃菌根化栽培及后续研究提供基础数据与支持。

Using a Ericoid Mycorrhiza(ERM) fungi strain with excellent drought resistance isolates inoculate to Rhododendron annae seedlings, then under different water stress conditions , the physiological mechanism of drought resisting were studied from Cellular and physiological metabolism two levels. To evaluate morphological changes of the mycorrhizal roots and plant cells with drought-resistant of Rhododendron annae seedling we research the mycorrhizal microstructure, plant cell fine structure and mycorrhizal colonisation rate index. Measure and probe the contents of endogenous hormones, photosynthetic characters especially the absorption and distribution of the photosynthetic product element, osmotic regulation, autioxidant enzyme activities. The project is to test the hypothesis that drought resistance increased of mycorrhizaled Rhododendron annae seedlings are because of ERM can rapid accumulation, promote change of plant hormones（especially the drought signal——abscisic acid，ABA）；At the same time, or in turn, influence control factors such as photosynthesis, antioxidant enzyme activity, thereby adjusting the physiological and biochemical reaction of the plants in response to drought stress. The significance of this project research is to provide the theoretical foundation to domestication , water-saving culture and landscape application with R. annae for example.At the same time, provide basic data for the mycorrhizal cultivation and follow-upresearch for alpine evergreen Rhododendron as well.