本研究组发现吸入麻醉患者发生术后学习记忆能力下降可持续到术后3个月甚至更久；SD大鼠异/七氟烷麻醉后这种影响可持续到数天，并与海马Aβ、Tau蛋白变化与表达有密切关联。由此推测异/七氟烷导致的学习记忆损伤与海马的生物学功能有关。为探索其机制，本研究通过新生大鼠间断重复吸入麻醉的整体和离体模型，以七氟烷为代表，从蛋白组和电生理水平研究哺乳期、幼年期、成年期和老年期学习记忆变化。利用Morris水迷宫实验、MRI、分子生物学技术及全细胞膜片钳技术，观察不同时期行为学、海马体积、海马和脑脊液（CSF）中Aβ、Tau蛋白、早老蛋白（PS）、ApoE基因及多态性、海马神经元电生理变化，揭示七氟烷麻醉后学习记忆变化及其机制；并进一步行临床评估认知水平，确证间断多次吸入七氟烷患者CSF中Aβ、Tau蛋白和ApoE的变化。旨在为临床诊断和治疗麻醉后学习记忆能力障碍提供理论基础和生物学标志物。

Our previous researches proved, inhalation anesthesia may lead to a decline in the ability of learning and memory in patients, which can last for 3 months or even longer after surgery. This phenomenon occurred in isoflurane or sevoflurane anesthetized Sprague-Dawley(SD) rats，and lasted several days，which is closely associated with hippocampal Aβ, Tau protein over turn and expression. Thus, we speculated that changes of learning and memory resulted from sevoflurane are closely related to the biological function of the hippocampus. In order to explore the mechanism, we have established newborn rat in vivo model and in vitro model, which are intermittently repeated inhalation anesthesia. From the proteome and electrophysiological level, research the learning and memory changes after sevoflurane anesthesia, during the lactation, infancy, adulthood and aged period in rats. By using the Morris water maze test, MRI, molecular biology techniques and whole-cell patch-clamp technique, we observed behavior change of rats, hippocampal volume, hippocampus and cerebrospinal fluid (CSF) of Aβ, Tau protein and Presenile protein ( PS ) , ApoE gene polymorphism , hippocampal neurons physiological changes at different periods. Out come of our study will reveal the mechanism of learning and memory changes after sevoflurane anesthesia. Further clinical studies are needed to assess the cognitive level, conclusive evidence the changes of Aβ, Tau protein and ApoE in cerebrospinal fluid of patients, after intermittent inhaled sevoflurane. To provide theoretical bases and biological markers for clinical diagnosis and treatment in learning and memory abilities obstacles resulted from inhalation anesthesia.