集调控病灶核心区再生微环境和强化内源性修复为一体的干细胞工程化治疗系统用于脑卒中修复和再生研究

本研究针对“组织器官再生修复的有序调控与干预策略”开展研究。干细胞移植疗法因其特有的神经保护及促进神经血管修复和再生的能力，成为脑卒中治疗的新兴手段，但其疗效仍受限，集中体现在：现有策略均无法安全有效地对病灶核心区高水平活性氧和炎性因子进行调控，从而大幅提高干细胞的存活率；亦缺乏全阶段改善病灶核心区再生微环境和促进内源性再生修复的有效策略。本项目拟在前期研究基础上，结合生物纳米技术、细胞表面工程、细胞膜仿生技术和基因工程等方法，设计一种集调控卒中损伤核心区再生微环境和强化内源性修复为一体的干细胞工程化纳米屏障系统，通过静脉注射的移植方法，高度靶向损伤核心区，并高效缓冲损伤核心区高水平炎性因子和活性氧对内/外源性干细胞和周围细胞的损伤，全阶段促进内源性修复和再生。该项目为脑卒中的干细胞移植疗法提供安全、一体化的治疗新策略，也为其他组织器官再生修复提供新思路，具有较高研究价值和临床转化前景。

Our research will focus on the project ‘Orderly regulation and intervention strategy for tissue and organ regeneration and repair’. Stem cell transplantation therapy has become an emerging treatment of stroke due to its unique neuroprotection and ability to promote neurovascular repair and regeneration. However, its efficacy is still limited, which is mainly reflected in the following aspects: none of the existing treatment methods can safely and effectively regulate the high levels of reactive oxygen species and inflammatory factors in the core area of the lesion, so as to improve the survival rate of stem cells. And there is also a lack of effective treatment strategies to improve the regeneration microenvironment and promote endogenous regeneration and repair of stroke lesions at all stages. Based on our previous researches, this project intends to integrate bio-nanotechnology, cell surface engineering, cell membrane bio-mimic technology and genetic engineering to design a stem cell engineering ‘nano-barrier’ system with great ability to improve injury-core microenvironment and enhancing endogenous repair and regeneration. After intravenous transplantation, the engineered stem cells are expected to specifically migrate to the injury-core and effectively protect internal/external stem cells and surrounding cells from the high level inflammatory factors and reactive oxygen species in the damaged core area, and promote endogenous repair and regeneration in the whole stage. Collectively, here we propose a new safe and integrated treatment strategy for stem cell transplantation therapy for stroke, which provides new ideas for the regeneration and repair of other tissues and organs, and holds high research value and clinical transformation prospect.