不孕不育是当前面临的严重社会问题，其中男性不育占30%；深入揭示精卵识别及精卵融合的分子机制并发掘有效促进该过程的药物在当前有重要意义。前期我们研究证实：益精方能有效增加精子数量、提高精子活力。为了进一步揭示益精方在改善男性不育中的作用，我们提出假设：益精方具有促进精子表达卵子识别蛋白及CatSper 蛋白，进而促进精卵识别和精卵融合、提高受精率的功效。本项目拟利用小鼠，经益精方处理，分析其对精子体外受精能力的影响，并研究益精方对精子表达卵子识别蛋白Izumo1、HSPA2 以及参与顶体反应的CatSper 的表达水平的调节作用，并进一步解析Izumo1 和卵子表面Juno 的结合情况，以及cAMP、PKA、PKC 信号通路参与顶体反应的信号机制。其研究成果将揭示益精方改善精卵结合的功效，进一步丰富益精方用于治疗男性不育的的理论基础，为开发益精方的潜在临床价值提供实验依据。

Infertility is the serious social problems at present, and male Infertility accounts 30% of the total infertility. It has important significance to reveal the molecule mechanism of sperm-egg recognition and sperm-egg fusion.In the early days , we had confirmed that YIJINGFANG could significantly enhance human sperm numbers and motility.In order to reveal the mechanism of YIJINGFANG improved male infertility, we supposed that YIJINGFANG can increase the expression of sperm-egg recognition protein and CatSper protein, and then promote the fertility rate.We plan to intragastric administration with YIJINGFANG to mice, and then analyze the rate of in vitro fertilization of sperm, the expression of Izumo1 and HSPA2 of sperm-egg recognition protein, and the expression of CatSper protein which participate acrosome reaction, further to analyze combine together Izumo1 and Juno of egg surface, and the mechanism of cAMP-PKA-PKC signal path in the process of acrosome reaction.