人口爆炸和非计划妊娠是全世界共同面临的公共卫生问题，开发一种安全、高效、经济、可逆的男性避孕方法是当前生殖与避孕领域研究的焦点问题之一。富含半胱氨酸分泌蛋白1(Crisp1)是一种由附睾头部上皮细胞合成分泌的糖蛋白，通过与卵子表面的互补位点相作用参与精卵融合的一系列过程。我们在前期研究中成功的构建了重组小鼠Crisp1蛋白疫苗和DNA疫苗，这两种疫苗均可诱导雌、雄性小鼠产生特异性的抗体，并具有一定的抗生育效果。然而Crisp1抗体的产生与小鼠不育症之间是否具有直接的因果关系以及Crisp1抗体究竟通过何种机制影响小鼠生育功能尚不清楚。本项目结合前期工作，拟采用免疫球蛋白缺陷小鼠模型和被动免疫试验证实特异性抗体的产生是否为Crisp1避孕疫苗成功避孕所必须，并通过体外试验进一步阐明Crisp1避孕疫苗的抗生育作用机制，为开发出经济、安全、有效的男性避孕疫苗奠定基础。

The whole world is faced with a severe public health problem caused by population explosion and unplanned pregnancy. Developing a safe efficient reversible male contraceptive tool is one of the focuses in current field of reproduction and contraception. Cysteine-rich secretory protein 1 (Crisp1), a glycoprotein synthesized and secreted by epithelial cells in epididymis, plays an important role in sperm-egg fusion process through complementary sites on the egg surface.In our research, recombinant mouse Crisp1 protein vaccine and DNA vaccine were successfully constructed which could induce both male and female mice to produce specific antibodies. However, it is unclear whether there is a direct causal relationship between Crisp1 antibodies production and infertility in mice, neither is the mechanism how Crisp1 influenced mouse reproductive function. In this program, we aim to adopt immunoglobulin-deficient mice models as well as passive immunization experiments to confirm whether specific antibodies are essential for the immuno-contraceptive effect induced by Crisp1 contraceptive vaccine, and explore the anti-fertility mechanism of Crisp1 vaccine in vitro experiment. The final purpose of our program is to lay a foundation for developing an economic, safe and effective male contraceptive vaccine.