西瓜番茄红素代谢相关基因挖掘及主效基因定位的研究

番茄红素具有抗氧化、抗肿瘤、保护心脑血管等功效，其保健作用已被国内外医学界公认，颜色较深的西瓜果肉中含有较高番茄红素，因此，选育高番茄红素西瓜是品质育种的重要内容之一。本研究利用西瓜"LSW-177"（红色）与"COS"（白色）高代自交系作为亲本材料，配制F1、F2以及F3代重组自交系群体，通过高通量测序技术及生物信息学分析手段，挖掘PSY、PDS、LYC等与西瓜番茄红素代谢密切相关基因，研究其功能，了解西瓜番茄红素代谢过程中基因表达模式，发现相关编码基因；开发多态性SNP、InDel分子标记；结合转录组学研究方法与F3代果实番茄红素含量，构建西瓜遗传连锁图谱，精细定位西瓜番茄红素代谢主效基因，克隆并验证其功能；探明其关联网络并加以验证；揭示影响西瓜番茄红素积累的遗传机理，为高番茄红素西瓜育种提供有效基因资源及实用分子标记，为进一步选育高番茄红素西瓜新品种奠定理论与材料基础。

Lycopene has effects of antioxidant, anti-tumor, protecting the cardiovascular and cerebrovascular, and its health effects has been recognized by the medical profession both in China and abroad.The watermelon flesh of deep colour contains higher lycopene, therefor,It is one of important aspects of quality breeding to breed high-lycopene watermelon. high and low lycopene containing watermelon varieties: The high generation inbred lines watermelon of "LSW-177" (red) and "COS" (white) were used as parents to make F1, F2 and F3 generation recombinant inbred lines in this experiment.High-throughput sequencing technologies and bioinformatics tools will be employed to excavate lycopene metabolism related genes in watermelon and predict gene function, such as PSY, PDS and LYC to understand gene expression patterns in the metabolic processes of watermelon lycopene,find the related proteincoding genes.Develop polymorphism molecular markers on SNP and InDel. Combination of transcriptome research methods and F3 fruit lycopene content to construct the genetic linkage map of watermelon and locate major genes of watermelon lycopene metabolism, isolate major genes and identify its function.Explore their associated genes networks and verify them again and to reveal the genetic mechanism of watermelon lycopene accumulation. Provide effective genetic resources and useful molecular markers for high lycopene breeding in watermelon, lay the theoretical and material foundation for the further breeding of new varieties of high lycopene watermelon.