TaEPFL1基因在小麦花发育中的功能研究

小麦雄蕊同源转化为雌蕊突变体（HTS-1）是研究小麦花发育和杂交小麦难得的珍稀材料，具有重要的理论研究价值和应用价值。TaEPFL1是从HTS-1中分离的一个在雌蕊化雄蕊（PS）中表达量异常高的基因，其表达量约为正常雌蕊（P）的20倍，正常雄蕊（S）的15倍。将该基因转入拟南芥后发现转基因植株的花丝明显短于柱头，花丝与柱头比值在0.35-0.79之间，且转基因植株花药发育异常，不能产生花粉。本研究拟在上述研究基础上利用CRISPR/Cas9基因编辑、基因在小麦中过表达以及酵母双杂交等技术进一步分析TaEPFL1基因在小麦花发育中的功能。本研究将为进一步阐明小麦雄蕊同源转化为雌蕊的分子机制提供了科学依据。同时期望为小麦雄性不育系的筛选和推动小麦杂交育种做贡献。

Homologous transformation sterility-1 (HTS-1) is a novel pistillody mutant in common wheat that transforms all or parts of the stamens into pistils or pistil-like structures.that cannot produce pollen. Therefore, HTS-1 is undoubtedly valuable for the study of stamen development in wheat. Members of the EPIDERMAL PATTERNING FACTOR-LIKE (EPFL) family play diverse roles in plant growth and development, including the guidance of inflorescence architecture and pedicel length. In previous studies, we identified and characterized the EFPL gene TaEPFL1 from the wheat pistillody mutant HTS-1. Quantitative real-time RT-PCR analysis showed that the TaEPFL1 gene is expressed at an abnormally high level in pistillody stamens (PS), approximately 20-fold higher than that in pistils (P) and approximately 15-fold higher than that in stamens (S). Heterologous expression of the TaEPFL1 gene in Arabidopsis caused shortened filaments, and the ratio of filaments to stigma ranged from 0.35 to 0.79. Most of the siliques of transgenic plants were short, empty, unable to produce seeds, and some had only residual pedicels without siliques. This study aims to further analyze the role of TaEPFL1 gene in flower development of wheat using the CRISPR/Cas9, gene overexpression in wheat and yeast two-hybrid. This study will provide a scientific basis for further elucidating the molecular mechanism of homologous transformation of stamen into pistil in wheat. At the same time, it is expected to contribute to the screening of male sterile lines and the promotion of wheat cross breeding.