土壤微生物驱动的氮转化过程是整体氮循环的重要环节。全球变暖和氮输入增加是当前全球性生态问题，但这两个因子共同作用下湿地土壤氮循环功能菌群驱动的氮转化过程模式受到的影响及其机制如何尚不清楚。为回答上述问题，我们拟在七里海湿地和实验室进行模拟增温和增氮交互实验，研究不同增温和增氮条件下湿地土壤氮循环功能基因丰度及基因构成、特定氮循环功能基因类型的菌群群落构成及多样性的差异；探究增温和增氮两个因子在对湿地土壤氮循环功能菌群共同作用中的关系（协同、拮抗、或无显著交互作用）；明确湿地土壤氮循环功能菌群组成特征变化的主导环境因子；揭示增温和增氮对湿地土壤氮循环功能菌群驱动的氮转化过程模式的影响及其机制。为科学预测未来全球变暖和氮输入增加持续加剧情况下湿地土壤氮循环功能的变化趋势，预先制定管控措施，有效保护和合理利用湿地土壤氮循环功能微生物资源，更好地发挥其在湿地生态系统中的功能和作用提供理论依据。

Microbially-driven nitrogen transformation is an important part of the whole nitrogen cycling process. Global warming and nitrogen input increase are current global ecological issues, but the effects of warming and nitrogen input increase on nitrogen transformation modes mediated by soil microorganisms and the underlying mechanisms in wetland ecosystems remain unclear. In order to address this knowledge gap, we propose to conduct experiments to simulate global warming and increasing nitrogen input with full-factorial design in the field and lab respectively. We will study abundance and composition of the functional genes involved in nitrogen cycling processes, and community structure and diversity of microorganisms with specific nitrogen cycling related functional gene under different experimental conditions. We will also explore the interaction between warming and nitrogen addition, and the main environmental factors influencing the composition of microorganisms involved in nitrogen cycling. Thus, this study will reveal the effects of warming and nitrogen input increase on nitrogen transformation modes mediated by soil microorganisms and the underlying mechanisms in wetland ecosystems. The results of this study will provide the scientific basis for better prediction of changes in soil nitrogen cycling function in wetlands, and appropriate exploitation of nitrogen cycling functional microorganisms, thereby effectively exerting their functions in wetland ecosystems.