橡胶材小蠹识别巴西橡胶树挥发物的分子机制研究

天然橡胶产业是我国不可替代的民族产业，巴西橡胶树是人工栽培的重要产胶植物，橡胶材小蠹是钻蛀橡胶树干的重要害虫。本项目以橡胶材小蠹和巴西橡胶树为研究对象，采用气相－液相质普分析、昆虫电生理、RACE－PCR、二代转录组测序等技术，分离鉴定巴西橡胶树受机械损伤后皮层和木质部产生的挥发性物质、测试该类物质对橡胶材小蠹的行为活性、观察橡胶材小蠹触角感受器的结构类型、从触角中提出鉴定嗅觉相关基因，并对气味结合蛋白、化学感受蛋白的组织表达、结合特性及与配体的特异性结合机制等进行研究，以期揭示橡胶材小蠹选择巴西橡胶树干钻蛀、取食、栖息等致害机理，为后续研发引诱、或阻遏等化学生态调控技术有效治理橡胶材小蠹、保护生态环境、保障我国天然橡胶产业持续、健康发展提供理论支持。

The nature rubber industry is an irreplaceable national industry in China. Rubber tree Hevea brasiliensis Muell.Arg. is the most important cultivated plant for rubber production in the world. Xyleborus affinis is an important bore pest in rubber tree trunk. In the project, the molecular mechanism of reognition of volatilities in Hevea brasiliensis Muell.Arg. by Xyleborus affinis Eichhoff will be studied by the techniques of GC-MS, insect electrophysiology, RACE-PCR and second-generation transcriptome sequencing to separat and identify volatile compound which produce from mechanical injury phloem and xylem of Hevea brasiliensis tree trunk, and testing behavior activity of volatile compound to Xyleborus affinis, observing Xyleborus affinis antennal sensilla ultrastructural organization and types, and several kinds of putative olfactory genes in its antennae were identified by Illumina Mesiq transcriptome sequencing, then to study on tissue expression, binding characterization and key binding site of odorant binding proteins and chemosensory proteins. So that wishing to explain the damage mechanism of Xyleborus affinis selecting Hevea brasiliensis tree trunk to bore, feed and perch. The result will supply to chemical ecological regulation technique to entrap or stop Xyleborus affinis on rubber tree, safeguard entironment and development of natural rubber industry in China.