水稻全基因组i-motif结构的鉴定及其形成机制的研究

DNA胞嘧啶四联体（i-motif）结构通过其结构的形成及展开，对基因的表达进行调控，进而参与一系列重要生物学进程。与现阶段鸟嘌呤四联体（G-quadruplex, G4）结构相比较，i-motif结构在基因组学及植物生物学功能研究方面远远滞后，尤其是在其形成机制的表观遗传学分子基础方面，尚缺乏系统研究。本项目通过发展iM-IP-Seq的实验方法，在全基因水平检测不同环境pH条件下，鉴定野生型水稻及其甲基化修饰改变材料的i-motif结构的分布，并结合BS-Seq数据，解析野生型水稻i-motif结构的形成与环境pH及基因组甲基化修饰水平的关系。通过本项目的实施，一方面将有助于加深对i-motif功能的研究，为其基因表达调控的研究奠定基础，在i-motif的角度为基因表达调控的研究提供新思路；另一方面，其也为基于基因表达调控的植物表观遗传育种提供理论参考。

DNA i-motif is involved in a series of important biological processes, such as regulating gene expression, by structure folding and unfolding.Compared with g-quadruplex (G4) structure, i-motif structure is fall behind in the studies of genomics and plant biological functions, especially in the epigenetic molecular basis of its formation mechanism. In this research, iM-IP-Seq was developed to detect rice and its methylated modified materials i-motif distribution genome wide，which under different environmental pH conditions. Basing on BS-Seq data, the i-motif formation mechanism was analyzed, which depending on environmental pH and genome DNA methylation in rice.By proceed this project, one hand, it will help deepen the research of i-Motif biological function, lay a foundation of gene expression regulation study, and also provide new insight of the study on gene expression regulation from the perspective of i-motif. On the other hand, it also provides a theoretical reference for plant epigenetic breeding based on gene expression regulation.