MdSRF1基因调控苹果植株抗盐性的分子机理

砧木抗盐性是影响苹果在盐渍化地区生长发育和分布的重要因素。在前期研究中，通过分析抗盐矮砧G935和盐敏感矮砧SH6中差异表达基因，获得了一个长效显著应答盐胁迫的新基因MdSRF1，过表达苹果愈伤抗盐性增强，可能与苹果砧木抗盐性差异有关。为进一步分析MdSRF1基因参与抗盐的机理，拟首先通过发根农杆菌转基因体系，分别获得MdSRF1基因过表达和敲除表达根系的SH6、G935矮砧植株，分析MdSRF1基因调控的植株抗逆生理变化和RNA-Seq分析其调控的信号通路。再对比分析Na+组织分布、根系外排流速等运输特性和抗盐性，进行MdSRF1基因与抗盐性互补验证。然后通过酵母双杂交筛选MdSRF1互作蛋白，构建MdSRF1基因调控的植株抗盐信号通路。结合转基因植株的Na+运输相关基因应答检测和对Na+运输的调控路径，最终揭示MdSRF1基因调控苹果植株抗盐性的分子机理。

High-salt resistance of rootstock is an important factor affecting the growth, development and distribution of apples in salinized areas. In previous studies, through analyzing the differentially expressed genes in salt-resistant dwarfing rootstock G935 and salt-sensitive dwarfing rootstock SH6, a new long-term and significant response gene to high-salt stress, MdSRF1, was obtained, and enhanced salt resistance in over-expressed apple callus was observed. In order to further analyze the resistance mechanism of MdSRF1 gene, SH6 and G935 rootstock plants with MdSRF1 gene over-expression and knock-out expression roots through Agrobacterium rhizogenes transgenic system will be processed respectively, and to analysis the stress-resistant physiological changes of plants regulated by MdSRF1 gene and RNA-Seq analysis of the regulatory signal pathway. And then the transport characteristics of Na+ tissue distribution, root efflux velocity, etc. will be analyzed and performed for complementary verification of salt tolerance. The MdSRF1 interaction protein will be screened by yeast two-hybrid to construct a salt-tolerance signaling pathway regulated by the MdSRF1 gene. Overall, combined with the detection of Na+ transport-related genes in transgenic plants and the regulatory pathway of Na+ transport, the molecular mechanism of MdSRF1 gene regulating the salt tolerance of apple plants will be finally revealed.