植物根顶端分生组织内组蛋白分子伴侣NRP1/2参与的分子与遗传互作

分生组织内干细胞自我更新和后代细胞分化之间的平衡对于植物器官的生长至关重要，这一过程依赖于各分生组织细胞染色质结构的精准调控。组蛋白分子伴侣参与核小体的组装与去组装，对分生组织细胞快速增殖所需的染色质复制以及细胞命运决定所需基因表达的精准调控都起着极为重要的作用。NRP1/2是对组蛋白H2A-H2B有偏爱性的组蛋白分子伴侣。我们现有的工作基础显示，在拟南芥根顶端分生组织中，NRP1/2与包括其它组蛋白分子伴侣、特定组蛋白乙酰基转移酶以及染色质重塑因子在内的多个染色质相关蛋白有着复杂而特异的分子与遗传互作。本项目旨在综合运用分子、蛋白、细胞、遗传和生信等研究手段，以NRP1/2为切入点和连接点，系统性地研究不同活性的染色质相关蛋白在根顶端分生组织内细胞增殖、基因组稳定性、转录调控以及细胞命运决定等方面的功能关联和互作，深入探索植物根顶端分生组织发育在染色质层面上的调控机制。

A delicate balance between self-renewal of stem cells and cell differentiation of their progenies within meristems is crucial for the proper growth and development of plant organs, which largely relies on the precise modulation of corresponding chromatin structure. Histone chaperones participate in nucleosome assembly and disassembly by mediating histones-DNA interaction, and play important roles in chromatin replication required for rapid meristematic cell proliferation as well as transcriptional modulation required for cell fate determination. NRP1/2 are histone chaperones with preference to histone H2A-H2B. Our previous studies have revealed that NRP1/2 are implicated in molecular and genetic interplays within Arabidopsis root apical meristems with other genes encoding distinct chromatin-related factors, including specific histone chaperone, histone acetyltransferase and chromatin remodeling factors. In this project, we will focus on these interplays connected by NRP1/2 in the aspects of cell proliferation, chromatin genome integrity and stability, transcriptional modulation and cell fate determination by using molecular, protein, cellular, genetic and bioinformatics approaches, and aim to unravel further underlying regulatory mechanisms at the chromatin level in root apical meristem development.