组蛋白甲基化修饰受组蛋白甲基转移酶和组蛋白去甲基化酶动态调控，主要通过对基因转录调控影响植物生长发育等过程。果实中对组蛋白甲基化修饰酶的鉴定和功能研究鲜有报道。我们前期在番茄中鉴定了一个H3K4me3去甲基化酶JUMONJI7（SlJMJ7），超表达SlJMJ7延缓成熟，敲除则相反且RIN的表达受其影响，我们推测SlJMJ7通过对RIN的H3K4me3修饰参与成熟的调控。本项目利用遗传学、多组学、分子生物学等手段，揭示SlJMJ7对番茄成熟的调控机制。主要内容包括：（1）遗传学分析SlJMJ7在番茄成熟中的功能；（2）SlJMJ7介导的H3K4me3去甲基化对成熟相关基因的转录调控作用；（3）番茄成熟过程中SlJMJ7对RIN基因H3K4me3去甲基化修饰的动态调控机制。该研究有望从表观遗传调控的角度扩展对果实成熟机理的认识，为改良果实品质、延长贮藏保鲜时间提供理论依据。

Histone methylation modification, which has important roles in plant growth and development by gene transcription, is written by histone methyltransferases and erased by histone demethylases. The identification and functional research of histone methylation enzymes are rarely reported during fruit ripening. Recently, We identified a H3K4me3 demethylase, JUMONJI 7 (SlJMJ7), overexpression of SlJMJ7 delayed tomato fruit ripening, while knockdown SlJMJ7 promoted fruit ripening. It is important that the expression of RIN was regulated by SlJMJ7. We speculate that SlJMJ7 participates in fruit ripening by removing the H3K4me3 demethylation modification of RIN, but the regulatory mechanism remains to be revealed. Therefore, in this project, the mechanism underlying the regulation of fruit ripening by histone demethylation modification would be studied from the perspective of genetics, biochemistry and genomics. The main contents include: (1) Characterization of SlJMJ7 protein in tomato fruit by genetic analysis; (2) Mechanism of SlJMJ7-mediated H3K4 demethylation regulates the transcription of ripening-related genes; (3) Mechanism of SlJMJ7 demethylation modification of RIN in fruit ripening. Study of the basic mechanisms of ripening from the perspective of epigenetic modification would potentialize manipulation of ripening and provide a theoretical basis for the development of postharvest handling to prolong fruit storage and transportation period.