电针治疗IBD内脏痛伴焦虑疗效显著，但其中枢机制尚不明确。研究表明，vHPC、mPFC、vlPAG、NAC等中枢核团与疼痛及焦虑密切相关，核团之间形成的神经环路是否参与电针镇痛抗焦虑尚不清楚。我们前期研究发现，化学遗传激活vHPC-mPFC投射的谷氨酸能神经元活性可拮抗电针治疗IBD的镇痛抗焦虑效应。本项目拟在此基础上，采用TNBS建立IBD模型，首先探讨电针不同配伍腧穴对IBD小鼠痛感觉和焦虑的影响，筛选出电针治疗IBD的最佳配伍腧穴；然后运用c-fos cre；Ai3小鼠筛选电针发挥镇痛抗焦虑效应时所激活的脑区；最后研究电针抑制vHPC-mPFC投射的谷氨酸能神经元活性发挥镇痛抗焦虑效应，增强mPFC-vlPAG投射的谷氨酸能神经元活性产生镇痛效应，增强mPFC-NAC投射的谷氨酸能神经元活性产生抗焦虑效应，为阐明电针调控神经环路治疗IBD内脏痛伴焦虑的机制提供科学依据。

Electroacupuncture(EA) is effective in the treatment of visceral pain and anxiety in inflammatory bowel disease, but its central mechanism is unclear. Studies have shown that vHPC, mPFC, vlPAG, NAC and other central nucleis are closely related to pain and anxiety. It is not clear whether the neural circuits formed between nuclear groups are involved in EA analgesia and anti-anxiety. Our previous study found that chemogenetic activation of glutamate neuronal activity in vHPC-mPFC projection could antagonize the analgesic and anti-anxiety effects of EA in the treatment of IBD. On this basis, this project intends to establish IBD model with 2,4,6-trinitrobenzenestdfonic acid. Firstly, discuss the effects of different acupoints of EA on pain sensation and anxiety in IBD mice, selects the best compatibility acupoints for EA treatment of IBD. Then uses c-fos CRE; Ai3 mouse to study the activated brain area when EA exerts the analgesic and anti-anxiety effects. Finally, EA suppresses the glutamatergic neurons of vPFC-mPFC projection to produce the analgesic and anti-anxiety effects, enhances the glutamatergic neuron activity of mPFC-vlPAG projection to produce the analgesic effect, enhance the activity of glutamate-capable neurons of mPFC-NAC projection to produce anti-anxiety effect, so as to provide scientific basis for elucidating the mechanism of EA regulating nerve circuits in the treatment of IBD visceral pain and anxiety.