烧伤创面局部免疫炎症反应失控不但使创面加深、延迟愈合，而且也是导致组织修复再生紊乱、严重瘢痕增生的重要因素。常规手术去除坏死组织或采用药物抑制某一炎性信号、清除炎性介质等效果并不理想。本研究拟采用微型组织工程技术和原理，适度平衡创面局部免疫炎症反应、重建适于组织修复再生的微环境，为烧伤创面修复提供新的视角。在以往研究证明骨髓间充质干细胞（MSC）对免疫炎症反应具有独特双向调节效应的基础上，以组织工程化羊膜为微载体，三维立体快速扩增高生物活性MSC。进一步采用原位生物打印技术，将负载MSC的羊膜微粒精准移植于创面局部，既保证治疗靶点获得高剂量、高活性的干细胞，同时也为移植后的MSC提供适于存活、增殖的精细化立体空间。将从免疫炎症调控的主要环节（免疫炎症因子；中性粒细胞、巨噬细胞、T淋巴细胞趋化、表型转换等）深入探讨MSC双向调控、平衡创面局部免疫炎症状态、促进组织修复再生的效果。

The severe immune inflammatory response of the local wound has not only made the wound deepen and delayed healing, but also led to tissue regeneration disorder and severe scarring. The conventional method of removing necrotic tissue, using drugs to suppress an inflammatory signal and clear the inflammatory mediators are not ideal. This study intends to use micro-tissue engineering techniques to balance immune inflammatory response of local wound, reconstruct microenvironment suitable for tissue regeneration, and provides a new perspective for the repair of burn wound. Previous studies have indicated that bone marrow mesenchymal stem cells (MSC) have a unique two-way regulatory effect on immune inflammatory response, and use tissue engineered amniotic membrane as a microcarrier to rapid amplify the MSC with high biological activity by three-dimensional technology, and then accurate transplant the amniotic particles loaded with MSC into the wound by the in-situ bioprinting technology, which not only gained high dose and active stem cells for therapeutic target, but also provide MSC with three-dimensional space to survive and proliferate. The study will conduct further research into the effect of two-way regulation, balancing immune inflammatory status, and promoting tissue regeneration of MSC from the key links of immune inflammation regulation (immune inflammatory factors, neutrophils, macrophages, T lymphocyte chemotaxis, phenotype change, etc).