肾脏尿酸转运蛋白参与尿酸在肾小管的重吸收和分泌，影响尿酸的排泄，维持着机体尿酸的稳态, 其功能异常在高尿酸血症的发生发展中起着关键作用。前期研究发现源于芒果叶的一种天然产物芒果苷具有较强的降尿酸活性(专利号：ZL 2008 1 0058019.6)，其作用不仅与抑制尿酸生成有关，还与促进尿酸的排泄有关，然而，芒果苷内在的作用机制尚未阐明。因此，本项目将从促进尿酸排泄角度，采用基因转染、RT-PCR、Western Blot、同位素标记等技术在整体和分子水平研究芒果苷对肾脏尿酸转运蛋白(URAT1、GLUT9、OAT1、OAT3、ABCG2、MRP4等)基因表达和功能的调控作用，以进一步阐明芒果苷降尿酸作用的分子机制，为芒果苷的进一步研发提供理论和实验依据.

The urate transporters, involved in reabsorption and secretion of urate in kidney, are responsible for maintaining the homeostasis of blood urate. The abnormal expression of genes, together with the dysfunction of urate transporters, plays an important role in the development of hyperuricemia. In our previous work, it was found that mangiferin, a natural product from Mangifera indica L. had potential hypouricemic effect in hyperuricemic mice induced by uricase inhibitor potassium oxonate (ZL 2008 1 0058019.6) and this effect was related not only to inhibiting the production of urate, but also to enhancing the excretion of urate. However, the internal hypouricemic mechanism of mangiferin remains unclear. Therefore, in the present program, we are going to investigate the excretion of urate in the model of hyperuricemic animals and explore the related hidden mechanism, aiming at providing more fundamental information for the development of mangiferin as a clinically useful drug.