中医学认为肾主骨，肾与骨关系密切。肾性骨营养不良是肾间质纤维化（RIF）的重要并发症。Wnt/β-catenin信号通路活化介导调控RIF的骨代谢异常。前期的研究证实加味六味地黄汤通过抗氧化、抗微炎症状态、阻断Notch信号通路活化及抑制TGF-β1、CTGF、MMP-2发挥抗RIF作用，但是否能调节Klotho-FGF23轴、双向调控肾和骨的Wnt/β-catenin信号发挥保护作用，国内外未见相关报道。本课题将Wnt/β-catenin信号及Klotho-FGF23为切入点，应用免疫组化、western blot等观察加味六味地黄汤对血清钙磷代谢和iPTH水平、骨代谢标记物（FGF-23、RANKL和Osteocalcin）、肾和骨组织Klotho、Wnt/β-catenin信号分子及下游的c-myc、FN的影响，探讨加味六味地黄汤抗RIF和骨保护作用机制和靶点以及肾主骨理论的物质基础

It is considered that kinney could control bone in the Chinese traditional medcine, suggestting that there is closed connect between the kidney and bone.Renal osteodystrophy is an important complication in the Renal interstitial fibrosis（RIF）. The activation of Wnt/β-catenin pathway is associated with the regulation of the bone metabolic abnormalities in the RIF. Oue previons works have proved that JiaWeiLiuWeiDiHuang decoction play a role through anti-oxidization, anti-microinflammation station,blocking the activation of Notch signaling, restrainning the TGF-β1, CTGF, MMP-2. However,there is not reports at home and abroad about whether JiaWeiLiuWeiDiHuang decoction could adjust the Klotho-FGF23 axis, provide the bi-directional regulation of the Wnt/β-catenin pathway. In this study, both Klotho-FGF23 axis and the Wnt/β-catenin pathway are regard as the entry point .The effect of JiaWeiLiuWeiDiHuang decoction on.the serum Calcium phosphorus metabolism and the level of iPTH, the markers of bonemetabolism including FGF-23, RANKL and Osteocalcin, Klotho-FGF23 axis, the signal molecule in the Wnt/β-catenin pathway and its downstream l molecule about c-myc and FN through the way of Immunohistochemistry and western blot, discussing the protictive mechanism and target of JiaWeiLiuWeiDiHuang decoction on the anti-RIF and bone protective role, and the material basis of the kinney controlling bone.