鸡毒支原体（Mycoplasma galliseticum，M. gallisepticum）是引起鸡慢性呼吸道疾病的病原体，广泛存在于国内外的养禽场中，给养禽业造成巨大的经济损失。我们前期研究发现：鸡毒支原体重组GroEL蛋白能够粘附在鸡胚胎成纤维细胞（DF-1）的表面并诱导该细胞发生凋亡，通过GST pull-down技术，初步筛选到与GroEL蛋白互作的凋亡相关蛋白：鸡膜联蛋白A2（Annexin A2）。本项目拟在此基础上，进一步鉴定与鸡毒支原体GroEL蛋白相互作用的受体蛋白Annexin A2，并确定二者诱导DF-1细胞凋亡的信号通路，阐释鸡毒支原体的致病机制。其研究结果将为鸡毒支原体病的防控和疫苗的研发提供理论依据。

Mycoplasma gallisepticum (M. gallisepticum) is the most important pathogen which causes chronic respiratory disease in poultry. Our previous study showed that the restructuring GroEL protein of M. gallisepticum can adhesion on the surface of host cell DF-1 and induce the cell apoptosis, and the GST pull-down results demonstrated that the rGroEL protein can interaction with apoptosis related proteins: chicken membrane protein A2 (Annexin A2). In this study, the host intracellular proteins interacted with GroEL and the relevant signal pathway will be identified, which will provide theoretical basis for new vaccines development and the prevention and control of M. gallisepticum.