DNA条形码是通过一个标准DNA片段对物种进行快速准确鉴定的分子生物学技术，该技术在部分物种鉴别中已得到成功的应用。疫霉属卵菌是农林业中的植物重要病原菌，与动物、植物相比，针对疫霉菌开展的DNA 条形码研究十分有限，目前尚没有统一的疫霉菌DNA条形码。本项目拟以疫霉属中概念清晰的种为材料，通过比较COI、Ypt1、（mtSSU）rDNA、TEF1-α、ITS、β-tubulin、rbcL、matK、LSU和ITS2等9个基因的PCR 扩增成功率、测序效率、种内和种间变异、barcoding gap 和鉴定成功率等指标，从中筛选出可用于疫霉属卵菌鉴定的DNA条形码通用序列。研究结果将有助于疫霉属卵菌的简便、准确、快速识别，对制定植物卵菌病害防制对策具有重要意义，还可为其它真菌类群的分类与系统发育研究提供参考。

DNA barcoding is a technique for identifying and characterizing species of organisms using a short DNA region. It is becoming more and more attractive to biologists as a new method to aid in species identification. DNA barcoding has been successfully employed for species identification in diverse groups of organisms such as insects, fishes, birds and prokaryotes. Phytophthora is a genus of plant pathogens responsible for severe damage to crops, natural vegetation and forestry worldwide. However, a universal DNA barcode for genus Phytophthora not been formally established. Our knowledge on genus Phytophthora DNA barcoding is still limited compared with that on animals and plants. . The aim of the project is to determine the candidate sequences which can be used as DNA barcode to identify species in genus Phytophthora by screening out from eleven different DNA fragments sequences of COI, Ypt1, (mtSSU)rDNA, TEF1-α, ITS, β-tubulin, rbcL, matK, LSU, and ITS2 genes, and the PCR amplification, sequencing efficiency, differential intra- and inter-specific divergences, DNA barcoding gap and identification efficiency were evaluated.. It is expected that this project will absolutely solve the problem of species identification of genus Phytophthora. which will benefits relative studies in ecological investigation, disease management and molecular system phylogenetic of fungi or oomycetes..