复方LC09治疗化疗致手足综合征的机理研究

化疗药物所致手足综合征是临床常见的并发症，严重影响患者的生活质量和治疗疗效，目前西医尚无有效防治方法。经前期临床研究中研究证明，“活血通络、解毒生肌”复方LC09外用对手足综合征具有良好的疗效和安全性。但采用动物整体实验研究手足综合征，目前我国尚属空白。本项研究采用脂质体阿霉素诱导的手足综合征小鼠动物模型，通过痛行为学、表皮组织组织病理学（基底细胞凋亡、胶原纤维分布）、神经电生理学的观测，以及介导手足综合征的相关因子（IL-10,CINC3）表达，评价复方LC09外用对手足综合征的治疗效果。并通过5-Fu及阿霉素导致的手足综合征离体细胞模型，探讨外用中药的作用靶点和机理。为临床中医药外用治疗化疗手足综合征提供指导性的科学依据，并进一步丰富中医“络病”理论和“治未病”科学内涵。

Chemotherapy-induced hand-foot syndrome is a common clinical complication. This complication seriously reduces patients’ quality of life and curative effects of chemotherapy. In western medicine, there is still no effective treatment. Previous clinical trials showed that "huoxue tongluo jiedu shengji" herbs LC09 local administration could effectively relieve hand-foot syndrome. However, no research in China has reported animal experiment about hand-foot syndrome. In this research, effects of tongluo huoxue herbs local administration are evaluated in a mouse model of hand-foot syndrome induced by liposomal doxorubicin. Pain behavior tests, histopathologic examines (basal cell apoptosis, collagen fiber distribution) of epidermal tissue, neural electrophysiological observations and expression of related cytokines (IL-10,CINC3) mediating hand-foot syndrome are evaluated. At the same time, effects of tongluo huoxue herbs local administration are evaluated using in vitro models of hand-foot syndrome induced by 5-fu or doxorubicin. Targets and mechanisms of herbs are investigated. This research will provide scientific basis of LC09 herbs local administration in treating chemotherapy-induced hand-foot syndrome. TCM luobing theory and TCM theory of curing not ill are enriched.