近年来，初级纤毛在细胞生长和发育、细胞识别等方面的作用机制备受关注。我们在嘉庚蛸精子形成机制研究中观察到精细胞表面的初级纤毛，在研究kinesin-2家族基因ot-kif3a与ot-kif3b在精子形成过程中的时空表达特征时推测其与初级纤毛关系密切，并与精子中段形成相关。我们认为ot-KIF3A/3B蛋白参与初级纤毛内物质运输，参与精子同步发生及中段形成。但ot-KIF3A/3B怎样参与精子同步发生和中段形成尚不知晓。我们假设ot-KIF3A/3B可能通过参与初级纤毛中的信号通路而向邻近生精细胞传递同步发育信息，并可能作为动力源之一运载线粒体参与精子中段形成。本项目拟用免疫印迹、免疫沉淀、双光子荧光显微镜、免疫电镜、基因过表达、morpholino干涉及抗体阻断等方法，研究ot-KIF3A/3B在嘉庚蛸精子形成中的功能，结果将揭示ot-KIF3A/3B参与精子同步发生及中段形成可能途经。

Primary cilia play important roles in cellular growth, development, and identification. We found that primary cilia exist on the surface of spermatids during spermatogenesis of Octopus tankahkeei, and we also found that kinesin-2 family genes (ot-kif3a and ot-kif3b) may have close relationship with primary cilia when we were investigating the temporal expression pattern of the above two genes. Our opinion is that ot-KIF3A/3B may participate in transportation in primary cilia, and may also take part in mid-piece formation by transporting mitochondria. Up to date, the molecular mechanisms of how ot-KIF3A/3B participates this process remains unknown. Our hypothesis is that, ot-KIF3A/3B may be involved in primary cilia pathway by sending out signals for synchronous spermiogenesis, and ot-KIF3A/3B may also carrying mitochondria to the mid-piece. To test this hypothesis, we plan to use the following molecular methods to investigate the potential pathway for synchronous spermiogenesis, these methods include: western blot, two-photon fluorescence microscopy, immuno-electron microscopy, gene over expression, morpholino interference, immunoprecipitation, and antibody blocking.