在临床上经常遇到因视网膜损伤、变性等因素导致的患者视力严重受损的问题，目前的治疗手段除了延缓病情的发展，尚没有更加有效的替代治疗方法。目前很多关于视网膜再生方面的研究显示，视网膜Müller胶质细胞具有干细胞特性，可被诱导分化为其他类型的视网膜神经细胞，为视网膜再生提供了高效重组的自体细胞来源。多项研究发现内质网应激可以参与在多种细胞的分化过程中，并且对某些细胞的干细胞特性有一定影响。本课题即立足于这两点研究基础，采用视网膜Müller细胞条件性GRP78基因敲除鼠模型诱导Müller细胞内的内质网应激、应用TM诱导原代Müller胶质细胞内质网应激，以及PBA或TMAO抑制原代Müller胶质细胞内质网应激等方法，观察在不同的内质网应激条件下Müller胶质细胞向感光细胞和神经节细胞诱导分化的改变，旨在研究内质网应激在诱导视网膜Muller胶质细胞分化过程中的调控作用。

Some research revealed that retina Müller glia have kinds of stemness properities, which could be induced the differentiation to some other kinds of retinal neurone cells. These findings indicate that retina Müller glia have the potential to serve as one kind of high efficient self-derived cell source for the retinal regeneration. There are some studies suggest that ER stress is involved in the differentiation procedures of many kinds of cells, and can affect the stemness characteristics of some stem cells and progenitor cells. Our study is based on these two points. with the using of Müller cell conditional GRP78 knockout mouse model, TM treatment and PBA or TMAO treatment to change the ER stress of the Müller glia , we want to see the difference of primary Müller glia cells differentiation to photo receptor and ganglion cell under different ER stress conditions, We aim to reveal that enhanced ER stress can induce the differentiation of retina Muller glia through affecting their stemness characteristis, so that we can provide some scientific evidence for facilitating the retinal regeneration.