已证明造血干细胞（HSC）衰老与机体衰老及老年病、骨髓造血抑制甚至白血病有密切联系。故延缓HSC衰老对阐述衰老机制及防治相关疾病有重大意义。目前造血干细胞衰老的机制研究很少涉及细胞记忆角度—即当干细胞外环境短暂改变时，干细胞会记忆刺激，通过基因调控，永久性分化为特定成熟组织细胞。有报道“补血之君”熟地的寡糖能促进HSC增殖分化，结合我们前期的熟地寡糖中质量控制指标甘露三糖具有抗细胞衰老作用等相关结果，本课题将对HSC衰老的细胞记忆机制做以初探并研究甘露三糖的抗HSC衰老作用。本课题拟建立HSC衰老模型，研究甘露三糖对HSC衰老相关指标及细胞记忆蛋白中转录抑制因子PCG家族和转录激活因子TrxG家族的关键基因及靶基因表达的影响，并通过RNA干扰技术明确HSC衰老的关键基因。本课题旨在寻找能激活衰老干细胞的药物及其可能的生物学调控机制，从而为延缓衰老和防治老年病提供新的实验支持。

It has been proved that hematopoietic stem cells (HSC) aging is closely related to aging and geriatric diseases, bone marrow hematopoietic suppression and even leukemia, so anti-aging of HSC would have major significance to explore the aging mechanism and prevent and treat of related diseases. At present, the research about mechanism of hematopoietic stem cell aging rarely related to cellular memory —— when the external environment of stem cells changes briefly, stem cells will remember the stimulate, through gene expression regulation, permanent differentiates the particular organization cells. It has been reported oligosaccharide in Rehmannia which is the “the prince of hemopoietics” can promote the HSC proliferation and differentiation, based on our previous experimental results such as mannotriose, as the Quality control indicators of Rehmannia, could resist the aging of hippocampal neuron , this topic will explore HSC aging mechanism from cellular memory angle and study the influence of manninotriose to HSC aging. In the topic, establlish aging HSC model in vitro, stem cell aging related conventional indicator, cellular gene expression of the key cell memory protein in transcription inhibitors PCG and transcription activator TrxG were detect ed to observe the changes in aging HSCs and the effect of mannotriose on the oboved indicator. We also use RNAi technology to make clear the key gene. The study aimed at developing new drugs which can resisit stem cells aging and its possible biological mechanism, and then providing a experimental basis for anti-aging and prevention and treatment of geriatric diseases.