基于脂代谢探讨蚊虫自噬调控wolbachia密度的作用机制

内生菌wolbachia的密度决定其诱导的抗病原能力。自噬作为真核生物抗胞内病毒和细菌的一个重要的防御机制，有研究发现它是调控wolbachia密度的关键因素，但其具体的作用机制未明。申请者前期工作发现，wolbachia感染可诱导蚊虫唾液腺内多个脂代谢相关基因上调。基于哺乳动物的研究发现，自噬可调控胞内脂代谢，而胞内脂滴可促进自噬的发生，据此我们提出假说，wolbachia诱导的自噬可能通过干扰细胞内的脂代谢而影响其密度。为此本课题拟从蚊虫细胞、组织水平研究wolbachia诱导的自噬与其密度关系，从组学角度明确wolbachia对蚊虫脂类代谢的影响，进而探讨在自噬被激活或抑制条件下，胞内脂代谢与wolbachia密度的关系，初步阐明脂代谢在蚊虫自噬调控wolbachia密度中的作用机制，为以wolbachia为生物防制剂高效、可持续控制蚊媒病提供理论依据。

The ability of wolbachia-medicated inhibition of viruses depends on its density. Autophagy is an important and well-conserved mechanism against virus and bacteriae in eukaryotic cells. And it has been found to be a key factor to modulate wolbachia density.But the mechanism is unclear. Previous work by applicant showed multiple lipid metabolic genes were up-regulated in mosquito salivary gland by wolbachia. Based on the conclusions from mammals, autophagy can modulate lipid metabolism and lipid droplet can modulate autophagosome biogenesis, we proposed the scientific hypothesis that mosquito autophagy might modulate lipid metabolism to influence wolbachia density. So this project will be performed to study the relationship between autophagy induced by wolbachia and its density in vitro and invivo, to clarify the change of lipid metabolism induced by wolbachia from the omics, and to investigate the relationship between lipid metabolism and wolbachia density when autophagy is activated or inhibited.Based on these results, it could be illuminated the role of lipid metabolism on mosquito autophagy modulated wolbachia density; it also could provide theoretical basis for wolbachia as a biocontrol agent to control arbodiseases efficently and sustainably.