胡麻是我国重要的油料作物及经济作物，胡麻籽粒中的生氰糖苷对人和动物的健康具有潜在的威胁。因此，明确胡麻生氰糖苷生成机制，降低胡麻籽粒中生氰糖苷的含量具有重要的意义。课题组前期研究中克隆得到9个胡麻CYP79基因，并初步证实其与生氰糖苷生成直接相关；本研究拟在此基础上设计gRNA，构建CRISPR敲除载体，通过胡麻遗传转化，研究CYP79基因的生物学功能；遗传转化阳性株系与阴性对照转录组比较分析，明确CYP79基因的调控网络；通过转化后代的遗传学及表型分析，获得籽粒中含量低或者不含生氰糖苷的胡麻优良中间育种材料。本研究结果不仅阐明CYP79基因的功能、遗传效应和作用机制，也为胡麻育种中生氰糖苷的遗传改良提供重要的基因资源。

Flax is very important oil crops and cash crops in China, but the existing of cyanogenic glucosides in flaxseed have potential threat to the health of human and animals. Therefore, understanding the generation mechanism and decreasing the content of cyanogenic glucosides in flaxseed has important significance. Our project team have cloned nine CYP79 genes in the prophase research, which directly regulate the generation of cyanogenic glucosides. On the basis of this results, we plan to design gRNA and construct CRISPR knockout vectors. We expect to confirm the biology function of CYP79 genes through genetic transformation of flax. Transcriptomic analysis of positive and negative control lines will be used to clear out the regulation network of CYP79 . Through genetic and phenotypic analysis of transgenic offsprings, We hope to gain excellent middle breeding materials of flax with low content of cyanogenic glucosides or without cyanogenic glucosides. This study will elucidate the function, genetic effect and mechanism of CYP79 genes and offer important genetic resources for genetic improvement of cyanogenic glucosides in flax breeding.