基于“肠道菌群-宿主代谢”途径的半夏泻心汤调控T2DM-IR作用机制研究

胰岛素抵抗是2型糖尿病、肥胖等多种代谢性病发生的共同土壤。“肠道菌群-宿主代谢”在胰岛素抵抗中扮演着重要角色。本课题拟在前期研究的基础上，以半夏泻心汤为代表的“助脾散精”法能影响“肠道菌群-宿主代谢”途径进而改善胰岛素抵抗为假说，选择GK大鼠为2型糖尿病模型，采用基因组学、代谢组学及分子生物学等技术手段，探索半夏泻心汤对2型糖尿病大鼠肠道菌种菌属、肠道菌群-宿主代谢产物、胃肠道黏膜形态和屏障变化及氧化应激、细胞凋亡及其相关基因等方面的影响，揭示半夏泻心汤改善胰岛素抵抗、稳定和调节血糖的科学内涵，为“助脾散精”法的深入研究奠定基础。

Insulin resistance is one of the most important common pathophysiological basis with many diseases, such as obesity and diabetes etc. "Intestinal flora host metabolism" plays an important role in IR. Based on the previous research, this topic select T2DM rats as model, and take the method of "Zhupi Sanjing" can affecting the "intestinal flora host metabolism" and improving insulin resistance as a hypothesis to observe the effects of Banxia Xiexin Decoction on intestinal flora, intestinal flora, host metabolism, gastrointestinal mucosal morphology and barrier, oxidative stress, apoptosis and related genes in T2DM rats, prompt the scientific connotation of Banxia Xiexin Decoction in improving insulin resistance, and lay the foundation for the further study of the method of "Zhupi Sanjing".